التفاصيل البيبلوغرافية
| العنوان: |
yggS Encoding Pyridoxal 5′-Phosphate Binding Protein Is Required for Acidovorax citrulli Virulence |
| المؤلفون: |
Wang, Yuanjie, Zhao, Yuqiang, Xia, Liming, Chen, Lin, Liao, Yajie, Chen, Baohui, Liu, Yiyang, Gong, Weirong, Tian, Yanli, Hu, Baishi |
| المساهمون: |
National Natural Science Foundation of China |
| المصدر: |
Frontiers in Microbiology ; volume 12 ; ISSN 1664-302X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
Microbiology (medical), Microbiology |
| الوصف: |
Bacterial fruit blotch, caused by seed-borne pathogen Acidovorax citrulli , poses a serious threat to the production of cucurbits globally. Although the disease can cause substantial economic losses, limited information is available about the molecular mechanisms of virulence. This study identified that, a random transposon insertion mutant impaired in the ability to elicit a hypersensitive response on tobacco. The disrupted gene in this mutant was determined to be Aave_0638 , which is predicted to encode a YggS family pyridoxal phosphate-dependent enzyme. YggS is a highly conserved protein among multiple organisms, and is responsible for maintaining the homeostasis of pyridoxal 5′-phosphate and amino acids in cells. yggS deletion mutant of A. citrulli strain XjL12 displayed attenuated virulence, delayed hypersensitive response, less tolerance to H 2 O 2 and pyridoxine, increased sensitivity to antibiotic β-chloro-D-alanine, and reduced swimming. In addition, RNA-Seq analysis demonstrated that yggS was involved in regulating the expression of certain pathogenicity-associated genes related to secretion, motility, quorum sensing and oxidative stress response. Importantly, YggS significantly affected type III secretion system and its effectors in vitro . Collectively, our results suggest that YggS is indispensable for A.citrulli virulence and expands the role of YggS in the biological processes. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fmicb.2021.783862 |
| DOI: |
10.3389/fmicb.2021.783862/full |
| الإتاحة: |
https://doi.org/10.3389/fmicb.2021.783862Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.CFE25AC0 |
| قاعدة البيانات: |
BASE |
