The roles of lipids and nucleic acids in HIV-1 assembly
| العنوان: | The roles of lipids and nucleic acids in HIV-1 assembly |
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| المؤلفون: | Ayna Alfadhli, Eric Barklis |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, Vol 5 (2014) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Microbiology (medical), Gag, lcsh:QR1-502, RNA, Review Article, Biology, Bioinformatics, Microbiology, Virus, lcsh:Microbiology, matrix, 3. Good health, Cell biology, chemistry.chemical_compound, Membrane, Capsid, chemistry, PI(4.5)P2, lipid, Nucleic acid, Function (biology), DNA, Intracellular |
| الوصف: | During HIV-1 assembly, precursor Gag (PrGag) proteins are delivered to plasma membrane (PM) assembly sites, where they are triggered to oligomerize and bud from cells as immature virus particles. The delivery and triggering processes are coordinated by the PrGag matrix (MA) and nucleocapsid (NC) domains. Targeting of PrGag proteins to membranes enriched in cholesterol and phosphatidylinositol-4,5-bisphosphate (PI[4,5]P2) is mediated by the MA domain, which also has been shown to bind both RNA and DNA. Evidence suggests that the nucleic acid-binding function of MA serves to inhibit PrGag binding to inappropriate intracellular membranes, prior to delivery to the PM. At the PM, MA domains putatively trade RNA ligands for PI(4,5)P2 ligands, fostering high affinity membrane binding. Triggering of oligomerization, budding and virus particle release results when NC domains on adjacent PrGag proteins bind to viral RNA, leading to capsid (CA) domain oligomerization. This process leads to the assembly of immature virus shells in which hexamers of membrane-bound MA trimers appear to organize above interlinked CA hexamers. Here we review the functions of retroviral MA proteins, with an emphasis on the nucleic acid binding capability of the HIV-1 MA protein, and its effects on membrane binding. |
| تدمد: | 1664-302X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde2d7024245a977f69f16ac6510cd26Test https://pubmed.ncbi.nlm.nih.gov/24917853Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dde2d7024245a977f69f16ac6510cd26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
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