Pleural Fluid GSDMD Is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion
| العنوان: | Pleural Fluid GSDMD Is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion |
|---|---|
| المؤلفون: | Qin Hu, Dandan Li, Lijing Zhou, Pu Li, Li Jun Zhang, Yuan Yuan, Xiaolan Zhou, Xuemei Peng, Qing Zhao, Hong Chen, Jing Shi, Bo Wang, Weixian Chen, Liang Duan |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, Vol 12 (2021) |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), medicine.medical_specialty, Pleural effusion, nucleated cells, Gastroenterology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Adenosine deaminase, Tuberculosis diagnosis, pleural effusion, Lactate dehydrogenase, Internal medicine, Medicine, Malignant pleural effusion, 030304 developmental biology, Original Research, 0303 health sciences, biology, Receiver operating characteristic, business.industry, pyroptosis, medicine.disease, QR1-502, chemistry, 030220 oncology & carcinogenesis, biology.protein, Biomarker (medicine), biomarker, Differential diagnosis, GSDMD, business |
| الوصف: | ObjectiveGasdermin D (GSDMD), controlling pyroptosis in cells, has multiple physiological functions. The diagnostic role of GSDMD in pleural effusion (PE) remains unknown.MethodsSandwich ELISA kits that we developed were applied to measure the level of GSDMD for 335 patients with a definite cause of PE, including transudative PE, tuberculous pleural effusion (TPE), parapneumonic pleural effusion (PPE), and malignant pleural effusion (MPE). The diagnostic accuracy of Light’s criteria vs. the new marker GSDMD was performed. Clinical follow-up of 40 cases of PPE was conducted and divided into efficacy and non-efficacy groups according to the therapeutic outcome. Nucleated cells (NCs) in PE were isolated and further infected with bacteria to verify the cell source of GSDMD.ResultsThe diagnostic accuracy of GSDMD for the diagnosis of PE were 96% (sensitivity) and 94% (specificity). The receiver operating characteristic (ROC) curve indicated that GSDMD can be an efficient biomarker for the differential diagnosis of transudative PE and other groups (all AUC > 0.973). Noteworthily, the highest AUC belonged to tuberculosis diagnosis of 0.990, and the cut-off value was 18.40 ng/mL. Moreover, the same cut-off value of PPE and MPE was 9.35 ng/mL. The combination of GSDMD, adenosine deaminase (ADA), and lactate dehydrogenase (LDH) will further improve the diagnostic efficiency especially between TPE and PPE (AUC = 0.968). The AUC of GSDMD change at day 4, which could predict the therapeutic effect at an early stage, was 0.945 (P < 0.0001). Interestingly, bacterial infection experiments further confirm that the pleural fluid GSDMD was expressed and secreted mainly by the NCs.ConclusionGSDMD and its combination are candidates as a potentially novel biomarker not only to separate PEs early and effectively, but also monitor disease progression. |
| اللغة: | English |
| تدمد: | 1664-302X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8229eec5644e622bbd1057500233cbdeTest http://europepmc.org/articles/PMC8215111Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8229eec5644e622bbd1057500233cbde |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
|---|