التفاصيل البيبلوغرافية
| العنوان: |
Stabilization of a Broadly Neutralizing Anti-Chikungunya Virus Single Domain Antibody |
| المؤلفون: |
Liu, Jinny L., Webb, Emily M., Zabetakis, Dan, Burke, Crystal W., Gardner, Christina L., Glass, Pamela J., Legler, Patricia M., Weger-Lucarelli, James, Anderson, George P., Goldman, Ellen R. |
| المساهمون: |
Defense Threat Reduction Agency |
| المصدر: |
Frontiers in Medicine ; volume 8 ; ISSN 2296-858X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2021 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
General Medicine |
| الوصف: |
A single domain antibody (clone CC3) previously found to neutralize a vaccine strain of the chikungunya virus (PRNT 50 = 2. 5 ng/mL) was found to be broadly neutralizing. Clone CC3 is not only able to neutralize a wild-type (WT) strain of chikungunya virus (CHIKV), but also neutralizes WT strains of Mayaro virus (MAYV) and Ross River virus (RRV); both arthralgic, Old World alphaviruses. Interestingly, CC3 also demonstrated a degree of neutralizing activity against the New World alphavirus, Venezuelan equine encephalitis virus (VEEV); albeit both the vaccine strain, TC-83, and the parental, WT Trinidad donkey strain had PRNT 50 values ~1,000-fold higher than that of CHIKV. However, no neutralization activity was observed with Western equine encephalitis virus (WEEV). Ten CC3 variants designed to possess a range of isoelectric points, both higher and lower, were constructed. This approach successfully identified several lower pI mutants which possessed improved thermal stabilities by as much as 10°C over the original CC3 (T m = 62°C), and excellent refolding abilities while maintaining their capacity to bind and neutralize CHIKV. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fmed.2021.626028 |
| DOI: |
10.3389/fmed.2021.626028/full |
| الإتاحة: |
https://doi.org/10.3389/fmed.2021.626028Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.7C170234 |
| قاعدة البيانات: |
BASE |
