Cell-Free Mitochondrial DNA in the CSF: A Potential Prognostic Biomarker of Anti-NMDAR Encephalitis
| العنوان: | Cell-Free Mitochondrial DNA in the CSF: A Potential Prognostic Biomarker of Anti-NMDAR Encephalitis |
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| المؤلفون: | Honghao Wang, Dong Zheng, Hai-Ying Shen, Yu Peng, Jun Zhang, Xiaomei Zhang, Suyue Pan, Teng Ji |
| المصدر: | Frontiers in Immunology, Vol 10 (2019) Frontiers in Immunology |
| بيانات النشر: | Frontiers Media S.A., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, anti-NMDAR encephalitis, Polymerase Chain Reaction, 0302 clinical medicine, Cerebrospinal fluid, Immunology and Allergy, Medicine, Anti-N-Methyl-D-Aspartate Receptor Encephalitis, biology, Interleukin, Middle Aged, Brief Research Report, Prognosis, Interleukin-10, Mitochondria, Interleukin 10, Real-time polymerase chain reaction, IL-10, Tumor necrosis factor alpha, Female, Cell-Free Nucleic Acids, Encephalitis, Adult, lcsh:Immunologic diseases. Allergy, Mitochondrial DNA, Immunology, modified rankin scale, DNA, Mitochondrial, cerebrospinal fluid, 03 medical and health sciences, Young Adult, Humans, Interleukin 6, cell-free mitochondrial DNA, IL-6, business.industry, Interleukin-6, Tumor Necrosis Factor-alpha, DNA, medicine.disease, cytokines, 030104 developmental biology, nervous system, TNF-α, biology.protein, business, lcsh:RC581-607, Biomarkers, 030215 immunology |
| الوصف: | Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is an autoimmune inflammatory brain disease that can develop a variety of neuropsychiatric presentations. However, the underlying nature of its inflammatory neuronal injury remains unclear. Mitochondrial DNA (mtDNA) is recently regarded as a damage-associated molecular pattern molecule (DAMP) that can initiate an inflammatory response. In the presenting study, we aimed to evaluate the levels of cell-free mtDNA in cerebrospinal fluid (CSF) of patients with anti-NMDAR encephalitis and to determine a potential role of cell-free mtDNA in the prognosis of anti-NMDAR encephalitis. A total of 33 patients with NMDAR encephalitis and 17 patients with other non-inflammatory disorders as controls were included in this study. The CSF levels of cell-free mtDNA were measured by quantitative polymerase chain reaction (qPCR). Cytokines including interleukin (IL)-6, IL-10, and tumor necrosis factor alpha (TNF-α) were measured by ELISA. The modified Rankin scale (mRS) score was evaluated for neurologic disabilities. Our data showed that the CSF levels of cell-free mtDNA and inflammation-associated cytokines were significantly higher in the patients with anti-NMDAR encephalitis compared with those in controls. Positive correlations were detected between the CSF levels of cell-free mtDNA and mRS scores of patients with anti-NMDAR encephalitis at both their admission and 6-month follow up. These findings suggest that the CSF level of cell-free mtDNA reflects the underlying neuroinflammatory process in patients with anti-NMDAR encephalitis and correlates with their clinical mRS scores. Therefore, cell-free mtDNA may be a potential prognostic biomarker for anti-NMDAR encephalitis. |
| اللغة: | English |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b3799c272f18509604735ecfa4ecb85Test https://www.frontiersin.org/article/10.3389/fimmu.2019.00103/fullTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6b3799c272f18509604735ecfa4ecb85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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