Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People
| العنوان: | Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People |
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| المؤلفون: | Dominique Huvent-Grelle, Julie Demaret, Alain Duhamel, Annie Sobaszek, Juliette Podvin, Fanny Vuotto, Laurence Bocket, Sophie Miczek, François Puisieux, Enagnon Kazali Alidjinou, Anne Goffard, Michael Hisbergues, Guillaume Lefèvre, Julien Labreuche, Arnaud Dendooven, Dominique Deplanque, Jacques Trauet, Bénédicte Corroyer-Simovic, Karine Faure, Myriam Labalette, Daniel Dreuil |
| المساهمون: | Université de Lille, Inserm, CHU Lille, Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017, Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286, Lille Neurosciences & Cognition (LilNCog) - U 1172, Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)], Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Hôpital Gériatrique Les Bateliers [Lille] (USLD - Nord), Pathogenèse virale du diabète de type 1 - ULR 3610 (Laboratoire de Virologie), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Centre d'Investigation Clinique - Innovation Technologique de Lille - CIC 1403 - CIC 9301 (CIC Lille), Santé Publique : épidémiologie et qualité des soins (EA 2694), Faculté de Médecine Henri Warembourg - Université de Lille-Centre d'Etudes et de Recherche en Informatique Médicale [Lille] (CERIM), Université de Lille, LillOA |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, 2021, Frontiers in Immunology, 12, pp.Article 778679. ⟨10.3389/fimmu.2021.778679⟩ Frontiers in Immunology, Vol 12 (2021) |
| بيانات النشر: | Frontiers Media SA, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, T cells response, Immunosenescence, T-Lymphocytes, [SDV]Life Sciences [q-bio], T cell, Immunology, Nutritional Status, Antibodies, Viral, Immunogenicity, Vaccine, Immune system, vaccine, medicine, Humans, Immunology and Allergy, BNT162 Vaccine, Original Research, Aged, 80 and over, Frailty, biology, SARS-CoV-2, business.industry, ELISPOT, mRNA vaccination, Antibody titer, COVID-19, RC581-607, Middle Aged, Antibodies, Neutralizing, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, SARS - CoV - 2, older people and ageing, |
| الوصف: | Long-term care facility (LTCF) older residents display physiological alterations of cellular and humoral immunity that affect vaccine responses. Preliminary reports suggested a low early postvaccination antibody response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to focus on the specific T-cell response. We quantified S1-specific IgG, neutralizing antibody titers, total specific IFNγ-secreting T cells by ELISpot, and functionality of CD4+- and CD8+-specific T cells by flow cytometry, after two doses of the BNT162b2 vaccine in younger and older people, with and without previous COVID-19 infection (hereafter referred to as COVID-19-recovered and COVID-19-naive subjects, respectively). Frailty, nutritional, and immunosenescence parameters were collected at baseline in COVID-19-naive older people. We analyzed the immune response in 129 young adults (median age 44.0 years) and 105 older residents living in a LCTF (median age 86.5 years), 3 months after the first injection. Humoral and cellular memory responses were dramatically impaired in the COVID-19-naive older (n = 54) compared with the COVID-19-naive younger adults (n = 121). Notably, older participants’ neutralizing antibodies were 10 times lower than the younger’s antibody titers (p + and IFNγ+IL-2+TNFα+ cells among specific CD4+ T cells, and the frequency of specific CD8+ T cells were lower in COVID-19-naive older participants than in COVID-19-naive young adults (p p = 0.0018, respectively). However, COVID-19-recovered older participants (n = 51) had greater antibody and T-cell responses, including IFNγ+ and IFNγ+IL-2+TNFα+-specific CD4+ T cells (p +-specific CD8+ T cells (p + and CD8+ T cells, in addition to impaired antibody response, and that specific studies are warranted to assess the efficiency of SARS-CoV-2 mRNA-based vaccines, as in other immunocompromised subjects. Our study also shows that, despite their physiological alterations of immunity, vaccination is highly efficient in boosting the prior natural memory response in COVID-19-recovered older people. |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::977037ef71749d297c7eec7d7e219b7fTest https://doi.org/10.3389/fimmu.2021.778679Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....977037ef71749d297c7eec7d7e219b7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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