Asymptomatic and Mild SARS-CoV-2 Infections Elicit Lower Immune Activation and Higher Specific Neutralizing Antibodies in Children Than in Adults
| العنوان: | Asymptomatic and Mild SARS-CoV-2 Infections Elicit Lower Immune Activation and Higher Specific Neutralizing Antibodies in Children Than in Adults |
|---|---|
| المؤلفون: | Maria Raffaella Petrara, Francesco Bonfante, Paola Costenaro, Anna Cantarutti, Francesco Carmona, Elena Ruffoni, Costanza Di Chiara, Marisa Zanchetta, Luisa Barzon, Daniele Donà, Liviana Da Dalt, Alessio Bortolami, Matteo Pagliari, Mario Plebani, Paolo Rossi, Nicola Cotugno, Paolo Palma, Carlo Giaquinto, Anita De Rossi |
| المساهمون: | Petrara, M, Bonfante, F, Costenaro, P, Cantarutti, A, Carmona, F, Ruffoni, E, Di Chiara, C, Zanchetta, M, Barzon, L, Dona, D, Da Dalt, L, Bortolami, A, Pagliari, M, Plebani, M, Rossi, P, Cotugno, N, Palma, P, Giaquinto, C, De Rossi, A |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, senescence, T-Lymphocytes, Antibodies, Viral, Severity of Illness Index, T-Lymphocytes, Regulatory, Serology, Immunology and Allergy, Medicine, Viral, Prospective Studies, Child, Neutralizing, Asymptomatic Infections, Original Research, B-Lymphocytes, B-Lymphocytes, Regulatory, biology, Middle Aged, Viral Load, Regulatory, Settore MED/38, Child, Preschool, Cytokines, Female, medicine.symptom, Antibody, Viral load, Senescence, Adult, Immunology, Tregs and Breg, Asymptomatic, Antibodies, Virus, immune activation, Plaque reduction neutralization test, Immune system, COVID-19 children, neutralizing antibodies (NAbs), Tregs and Bregs, Antibodies, Neutralizing, COVID-19, Humans, Lymphocyte Count, Pathogen-Associated Molecular Pattern Molecules, SARS-CoV-2, Preschool, business.industry, RC581-607, biology.protein, Immunologic diseases. Allergy, business |
| الوصف: | BackgroundThe immune response plays a pivotal role in dictating the clinical outcome in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected adults, but it is still poorly investigated in the pediatric population.MethodsOf 209 enrolled subjects, 155 patients were confirmed by PCR and/or serology as having coronavirus disease 2019 (COVID-19). Blood samples were obtained at a median of 2.8 (interquartile, 2.1–3.7) and 6.1 (5.3–7.2) months after baseline (symptom onset and/or first positive virus detection). The immune profiles of activation, senescence, exhaustion, and regulatory cells were analyzed by flow cytometry. Neutralizing antibodies (nAbs) were detected by a plaque reduction neutralization test. In available nasopharyngeal swabs at baseline, SARS-CoV-2 levels were quantified by digital droplet PCR (ddPCR).ResultsOverall, COVID-19 patients had higher levels of immune activation, exhaustion, and regulatory cells compared to non-COVID-19 subjects. Within the COVID-19 group, activated and senescent cells were higher in adults than in children and inversely correlated with the nAbs levels. Conversely, Tregs and Bregs regulatory cells were higher in COVID-19 children compared to adults and positively correlated with nAbs. Higher immune activation still persisted in adults after 6 months of infection, while children maintained higher levels of regulatory cells. SARS-CoV-2 levels did not differ among age classes.ConclusionsAdults displayed higher immune activation and lower production of anti-SARS-CoV-2 nAbs than children. The different immune response was not related to different viral load. The higher expression of regulatory cells in children may contribute to reduce the immune activation, thus leading to a greater specific response against the virus. |
| وصف الملف: | ELETTRONICO |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a81eb83a684ab5ddc34f65bcb1bbec7Test https://pubmed.ncbi.nlm.nih.gov/34659235Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5a81eb83a684ab5ddc34f65bcb1bbec7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
|---|