A Conserved TCRβ Signature Dominates a Highly Polyclonal T-Cell Expansion During the Acute Phase of a Murine Malaria Infection
| العنوان: | A Conserved TCRβ Signature Dominates a Highly Polyclonal T-Cell Expansion During the Acute Phase of a Murine Malaria Infection |
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| المؤلفون: | Natasha L. Smith, Wiebke Nahrendorf, Graeme J. M. Cowan, Philip J Spence, Jason P. Mooney, Joanne Thompson, Catherine Sutherland |
| المصدر: | Frontiers in Immunology Smith, N, Nahrendorf, W, Sutherland, C, Mooney, J, Thompson, J, Spence, P J & Cowan, G J M 2020, ' A Conserved TCRβ signature dominates a highly polyclonal T-cell expansion during the acute phase of a murine malaria infection ', Frontiers in Immunology, vol. 11, 587756 . https://doi.org/10.3389/fimmu.2020.587756Test Frontiers in Immunology, Vol 11 (2020) |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Immunologic diseases. Allergy, CD4-Positive T-Lymphocytes, T cell, Receptors, Antigen, T-Cell, alpha-beta, Immunology, malaria, Biology, Plasmodium, Plasmodium chabaudi, 03 medical and health sciences, 0302 clinical medicine, Immune system, T-cell, medicine, Immunology and Allergy, Animals, immune-repertoire, Receptor, Gene, 030304 developmental biology, Original Research, 0303 health sciences, T-cell receptor, single-cell, medicine.disease, biology.organism_classification, Virology, 3. Good health, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Polyclonal antibodies, Acute Disease, biology.protein, Female, lcsh:RC581-607, Malaria, TCR, Spleen, 030215 immunology |
| الوصف: | CD4+ αβ T-cells are key mediators of the immune response to a first Plasmodium infection, undergoing extensive activation and splenic expansion during the acute phase of an infection. However, the clonality and clonal composition of this expansion has not previously been described. Using a comparative infection model, we sequenced the splenic CD4+ T-cell receptor repertoires generated over the time-course of a Plasmodium chabaudi infection. We show through repeat replicate experiments, single-cell RNA-seq, and analyses of independent RNA-seq data, that following a first infection – within a highly polyclonal expansion – T-effector repertoires are consistently dominated by TRBV3 gene usage. Clustering by sequence similarity, we find the same dominant clonal signature is expanded across replicates in the acute phase of an infection, revealing a conserved pathogen-specific T-cell response that is consistently a hallmark of a first infection, but not expanded upon re-challenge. Determining the host or parasite factors driving this conserved response may uncover novel immune targets for malaria therapeutic purposes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7759279062c762af9f995dcd72286877Test http://europepmc.org/articles/PMC7719809Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7759279062c762af9f995dcd72286877 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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