التفاصيل البيبلوغرافية
| العنوان: |
Respiration-Locking of Olfactory Receptor and Projection Neurons in the Mouse Olfactory Bulb and Its Modulation by Brain State. |
| المؤلفون: |
Ackels, Tobias, Jordan, Rebecca, Schaefer, Andreas T., Fukunaga, Izumi |
| المصدر: |
Frontiers in Cellular Neuroscience; 7/16/2020, Vol. 14, p1-15, 15p |
| مصطلحات موضوعية: |
OLFACTORY bulb, NEURONS, NASAL mucosa, OLFACTORY receptors, SENSORY neurons, AIR flow, HYPERPOLARIZATION (Cytology), SENSE organs |
| مستخلص: |
For sensory systems of the brain, the dynamics of an animal's own sampling behavior has a direct consequence on ensuing computations. This is particularly the case for mammalian olfaction, where a rhythmic flow of air over the nasal epithelium entrains activity in olfactory system neurons in a phenomenon known as sniff-locking. Parameters of sniffing can, however, change drastically with brain states. Coupled to the fact that different observation methods have different kinetics, consensus on the sniff-locking properties of neurons is lacking. To address this, we investigated the sniff-related activity of olfactory sensory neurons (OSNs), as well as the principal neurons of the olfactory bulb (OB), using 2-photon calcium imaging and intracellular whole-cell patch-clamp recordings in vivo , both in anesthetized and awake mice. Our results indicate that OSNs and OB output neurons lock robustly to the sniff rhythm, but with a slight temporal shift between behavioral states. We also observed a slight delay between methods. Further, the divergent sniff-locking by tufted cells (TCs) and mitral cells (MCs) in the absence of odor can be used to determine the cell type reliably using a simple linear classifier. Using this classification on datasets where morphological identification is unavailable, we find that MCs use a wider range of temporal shifts to encode odors than previously thought, while TCs have a constrained timing of activation due to an early-onset hyperpolarization. We conclude that the sniff rhythm serves as a fundamental rhythm but its impact on odor encoding depends on cell type, and this difference is accentuated in awake mice. [ABSTRACT FROM AUTHOR] |
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