التفاصيل البيبلوغرافية
| العنوان: |
Euglena gracilis paramylon activates human lymphocytes by upregulating pro-inflammatory factors. |
| المؤلفون: |
Russo, Rossella, Barsanti, Laura, Evangelista, Valter, Frassanito, Anna M., Longo, Vincenzo, Pucci, Laura, Penno, Giuseppe, Gualtieri, Paolo |
| المصدر: |
Food Science & Nutrition; Mar2017, Vol. 5 Issue 2, p205-214, 10p |
| مصطلحات موضوعية: |
EUGLENA gracilis, LYMPHOCYTES, BETA-glucans, NATURAL immunity, IMMUNOCYTOCHEMISTRY, SONICATION |
| مستخلص: |
The aim of this study was to verify the activation details and products of human lymphomonocytes, stimulated by different β-glucans, that is Euglena paramylon, MacroGard®, and lipopolysaccharide. We investigated the gene expression of inflammation-related cytokines and mediators, transactivation of relevant transcription factors, and phagocytosis role in cell-glucan interactions, by means of RT- PCR, immunocytochemistry, and colorimetric assay. Our results show that sonicated and alkalized paramylon upregulates pro-inflammatory factors ( NO, TNF- α, IL-6, and COX-2) in lymphomonocytes. A clear demonstration of this upregulation is the increased transactivation of NF- kB visualized by immunofluorescence microscopy. Phagocytosis assay showed that internalization is not a mandatory step for signaling cascade to be triggered, since immune activity is not present in the lymphomonocytes that have internalized paramylon granules and particulate MacroGard®. Moreover, the response of Euglena β-glucan-activated lymphomonocytes is much greater than that induced by commercially used β-glucans such as MacroGard®. Our in vitro results indicate that linear fibrous Euglena β-glucan, obtained by sonication and alkaline treatment can act as safe and effective coadjutant of the innate immune system response. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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