An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk
| العنوان: | An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk |
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| المؤلفون: | Lili Li, Zonghao Yue, Jifei Yang, Zhongke Sun, Yinbiao Wang, Xiaofei Hu, Le He, Xingdong Yang |
| المصدر: | Food Analytical Methods. 12:2430-2437 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Detection limit, Chromatography, Strip test, Chemistry, Danofloxacin, 010401 analytical chemistry, 04 agricultural and veterinary sciences, 040401 food science, 01 natural sciences, Applied Microbiology and Biotechnology, High-performance liquid chromatography, Rapid detection, 0104 chemical sciences, Analytical Chemistry, Highly sensitive, 0404 agricultural biotechnology, medicine, Safety, Risk, Reliability and Quality, Safety Research, Food Science, medicine.drug |
| الوصف: | An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a competitive format. Quantitative detections of DAN-spiked samples using the ILFST gave a half inhibitory concentration (IC50) of 0.513 ng/mL and a limit of detection (LOD) of 0.092 ng/mL. Recoveries of DAN from spiked milk samples were from 80.0 to 95.1% within an assay (intra-assay) and from 87.0 to 89.8% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 2.09–9.93% and 2.42–12.07%, respectively. These data indicated that the ILFST was highly sensitive and reproducible. Detections of authentic milk samples using ILFST and high-performance liquid chromatography (HPLC) showed no significant differences between the two methods. Hence, the ILFST can be widely used for both qualitative and quantitative detection of DAN residues in milk samples. |
| تدمد: | 1936-976X 1936-9751 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::9ad70bab85bf3797fa9cfdcf647052e7Test https://doi.org/10.1007/s12161-019-01601-9Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........9ad70bab85bf3797fa9cfdcf647052e7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1936976X 19369751 |
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