دورية أكاديمية
Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring
| العنوان: | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
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| المؤلفون: | Lingxing Wang, Honghong Huang, Hongchao Cai, Yafang Chen |
| المصدر: | Folia Neuropathologica, Vol 55, Iss 4, Pp 317-324 (2017) |
| بيانات النشر: | Termedia Publishing House, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | maternal hypoxia, offspring, cerebral ischemia, hippocampus, disease susceptibility, Medicine |
| الوصف: | Introduction : Maternal hypoxia induces an adverse uterine environment and may induce long-term effects in offspring. This study investigated whether maternal hypoxia increases hippocampal cell vulnerability and exacerbates neurological impairments in adult rat offspring following ischemia. Material and methods : Pregnant Sprague-Dawley rats were randomly assigned to no maternal hypoxia or maternal hypoxia treatment groups. Adult male rat offspring were subjected to middle cerebral artery occlusion (MCAO). There were four groups: maternal + sham (MH + Sham), sham (Sham), maternal hypoxia + MCAO (MH + MCAO), and MCAO only (MCAO). Neurological deficits were evaluated. Hippocampal cell damage was observed by hematoxylin and eosin (HE) staining. Cell apoptosis in the hippocampus was detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining. Caspase-3, cytochrome c, Bax, and bcl-2 expression in the hippocampus was detected by Western blot. Results : More severe hippocampal cell damage was found in the MH + MCAO group than in the MCAO group. Additionally, neurological deficits, percentage of TUNEL positive cells, and expression of caspase-3, cytochrome c, and Bax in the hippocampus were significantly higher (p < 0.05), whereas bcl-2 expression was significantly lower (p < 0.05) in the MH + MCAO group compared to the MCAO group. Conclusions : These findings suggest that maternal hypoxia may exacerbate hippocampal cell apoptosis in rat offspring after MCAO via alterations in the expression of cytochrome c, caspase-3, Bax, and bcl-2, which ultimately affects ischemic stroke prognosis. To our knowledge, this is the first study demonstrating that maternal hypoxia increases hippocampal cell susceptibility to ischemia in adult rat offspring. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1641-4640 1509-572X |
| العلاقة: | https://www.termedia.pl/Maternal-hypoxia-increases-hippocampal-cell-susceptibility-to-ischemia-after-middle-cerebral-artery-occlusion-in-rat-offspring,20,31352,1,1.htmlTest; https://doaj.org/toc/1641-4640Test; https://doaj.org/toc/1509-572XTest |
| DOI: | 10.5114/fn.2017.72392 |
| الوصول الحر: | https://doaj.org/article/7a62abeb097c47d6a2e34a1266e8c890Test |
| رقم الانضمام: | edsdoj.7a62abeb097c47d6a2e34a1266e8c890 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16414640 1509572X |
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| DOI: | 10.5114/fn.2017.72392 |