التفاصيل البيبلوغرافية
| العنوان: |
Discovery of biomarkers of endometrial receptivity through a minimally invasive approach: a validation study with implications for assisted reproduction. |
| المؤلفون: |
Chan, Crystal1,2,3,4 cryssie.chan@mail.utoronto.ca, Virtanen, Carl5, Winegarden, Neil A.5, Colgan, Terence J.6, Brown, Theodore J.1,2,3, Greenblatt, Ellen M.1,4 |
| المصدر: |
Fertility & Sterility. Sep2013, Vol. 100 Issue i3, p810-817.e8. 0p. |
| مصطلحات موضوعية: |
*BIOMARKERS, *REPRODUCTIVE technology, *MENSTRUAL cycle, *GENE expression, *CELL separation, *LONGITUDINAL method |
| مستخلص: |
Objective: To determine whether a minimally invasive approach to sampling endometrial cells that can be applied during an active conception cycle can generate robust biomarker candidates for endometrial receptivity by genomewide gene expression profiling. Design: Longitudinal study comparing gene expression profiles of cells isolated from uterine aspirates collected during the prereceptive and receptive phases of a natural cycle. Setting: University-affiliated hospital. Patient(s): Healthy volunteers, ≤40 years of age, with regular menstrual cycles and no history of infertility. Intervention(s): One menstrual cycle monitored with urinary kits to identify the luteinizing hormone (LH) surge; uterine aspirations collected at LH + 2 days (LH + 2) and at LH + 7; endometrial biopsy obtained on LH + 7; RNA extraction from the cellular material for gene expression profiling, and differential gene expression validated by NanoString assay and cross-validated against a publically available data set. Main Outcome Measure(s): Differentially expressed genes between LH + 2 and LH + 7 samples. Result(s): NanoString assay validated 96% of the 245 genes found differentially expressed at LH + 7. Unsupervised hierarchical clustering of aspiration and biopsy samples demonstrated the concordance of the sampling methods. A predictor gene cassette derived by a shrunken centroid class prediction technique correctly classified the receptive phase within an external data set. Conclusion(s): Uterine aspiration, which can be performed during an active conception cycle, identified robust candidate biomarkers of endometrial receptivity, and will enable their validation by direct correlation with clinical outcomes. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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