Conservation of type III secretion system genes inBradyrhizobiumisolated from soybean
| العنوان: | Conservation of type III secretion system genes inBradyrhizobiumisolated from soybean |
|---|---|
| المؤلفون: | Séverine Siblot, Mélanie Lemunier, Sylvie Mazurier, Philippe Lemanceau, Alain Hartmann |
| المساهمون: | Microbiologie, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB) |
| المصدر: | FEMS Microbiology Letters FEMS Microbiology Letters, Wiley-Blackwell, 2006, 259 (2), pp.317-325. ⟨10.1111/j.1574-6968.2006.00290.x⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | DNA, Bacterial, Genotype, rhc genes, sinorhizobium, hrc genes, Microbiology, Bradyrhizobium, microorganisme du sol, law.invention, 03 medical and health sciences, law, Genetics, RELATION PLANTE-MICROORGANISME, Symbiosis, Molecular Biology, Gene, Phylogeny, Bradyrhizobium elkanii, Polymerase chain reaction, 030304 developmental biology, Southern blot, 0303 health sciences, Base Sequence, bradyrhizobium, biology, mesorhizobium, 030306 microbiology, Genetic transfer, biochemical phenomena, metabolism, and nutrition, Ribosomal RNA, biology.organism_classification, type III secretion system-T3SS, RNA, Bacterial, Phenotype, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Genes, Bacterial, RNA, Ribosomal, bacteria, Soybeans, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length |
| الوصف: | International audience; The distribution of rhcRST genes encoding the type III secretion system (T3SS) in a collection of Bradyrhizobium strains was characterized by PCR and Southern blot hybridization. The polymorphism of the corresponding sequences amplified by PCR was characterized by RFLP and sequencing together with those available in the databank. Genomic group I is characterized by the presence of Bradyrhizobium elkanii strains and group II by the presence of B. japonicum and B. liaoningense strains. Highly conserved T3SS-like genes were detected by PCR in all Bradyrhizobium strains isolated from soybean belonging to genomic group II, and in none of the strains belonging to genomic group I. These data were confirmed by Southern blot hybridization that further indicated the presence of sequences showing similarity to the rhcRST sequence in B. elkanii strains. The high level of conservation of rhcRST among Bradyrhizobia of genomic group II and sharing the same host-plant suggests that T3SS-like genes might have undergone horizontal genetic transfer within this genomic group. When considering the three Rhizobiaceae genera, a clear congruence was recorded between the rhcRST, rRNA gene and ITS sequences in bacteria harbouring sequences encoding T3SS, suggesting a relatively ancient emergence of the T3SS in these genera. |
| تدمد: | 1574-6968 0378-1097 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02ddc4dd772bfc72aa81353dc5ef1d83Test https://doi.org/10.1111/j.1574-6968.2006.00290.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....02ddc4dd772bfc72aa81353dc5ef1d83 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15746968 03781097 |
|---|