We studied the influence of specific growth conditions on the induction of β-oxidation enzymes and rate of microbiology proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbiology numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbiology biogenesis/assembly in constructed, conditional pas mutants.
