التفاصيل البيبلوغرافية
| العنوان: |
Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora. |
| المؤلفون: |
Manubens, Augusto1, Canessa, Paulo1, Folch, Carolina1, Avila, Marcela1, Salas, Loreto1, Vicuña, Rafael1 rvicuna@bio.puc.cl |
| المصدر: |
FEMS Microbiology Letters. Oct2007, Vol. 275 Issue 1, p139-145. 7p. 4 Graphs. |
| مصطلحات موضوعية: |
*MANGANESE, *LACCASE, *BASIDIOMYCETES, *FUNGAL genetics, *MESSENGER RNA, *MYCELIUM, *AROMATIC compounds, *METAL ions, *MICROBIOLOGY |
| مستخلص: |
The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0–10 μM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160–194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag+, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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