التفاصيل البيبلوغرافية
| العنوان: |
Maize polyamine oxidase: primary structure from protein and cDNA sequencing |
| المؤلفون: |
Tavladoraki, Paraskevi, Schininà, M.Eugenia, Cecconi, Francesco, Di Agostino, Silvia, Manera, Francesco, Rea, Giuseppina, Mariottini, Paolo, Federico, Rodolfo, Angelini, Riccardo |
| المصدر: |
FEBS Letters; April 1998, Vol. 426 Issue: 1 p62-66, 5p |
| مستخلص: |
The first complete amino acid sequence of a flavin‐containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis. A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription‐polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined. This cDNA contains information for a polypeptide chain of 500 amino acids. Its amino‐terminal sequence shows the typical features of secretion signal peptides. The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin‐containing monoamine oxidases are also discussed. |
| قاعدة البيانات: |
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