التفاصيل البيبلوغرافية
| العنوان: |
The E3 ubiquitin ligase MARCH3 controls the endothelial barrier. |
| المؤلفون: |
Leclair, Héloïse M.1,2,3, André-Grégoire, Gwennan1,2, Treps, Lucas3, Azzi, Sandy3, Bidère, Nicolas1,2,3, Gavard, Julie1,2,3 |
| المصدر: |
FEBS Letters. Oct2016, Vol. 590 Issue 20, p3660-3668. 9p. |
| مصطلحات موضوعية: |
*UBIQUITIN ligases, *ENDOTHELIAL cells, *CELL communication, *TIGHT junctions, *CELLULAR signal transduction |
| مستخلص: |
Cell-cell contacts coordinate the endothelial barrier function in response to external cues. To identify new mediators involved in cytokine-promoted endothelial permeability, we screened a si RNA library targeting E3 ubiquitin ligases. Here, we report that silencing of the late endosome/lysosomal membrane-associated RING- CH-3 ( MARCH3) enzyme protects the endothelial barrier. Furthermore, transcriptome analysis unmasked the upregulation of the tight junction-encoding gene occludin ( OCLN) in MARCH3-depleted cells. Indeed, MARCH3 silencing results in the strengthening of cell-cell contacts, as evidenced by the accumulation of junctional proteins. From a molecular standpoint, the FoxO1 forkhead transcription repressor was inactivated in the absence of MARCH3. This provides a possible molecular link between MARCH3 and the signaling pathway involved in regulating the expression of junctional proteins and barrier integrity. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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