التفاصيل البيبلوغرافية
| العنوان: |
Leukotriene B4 and lipoxin A4 are regulatory signals for neural stem cell proliferation and differentiation. |
| المؤلفون: |
Wada, Koichiro1 kwada@dent.osaka-u.ac-jp, Arita, Makoto2,3,4, Nakajima, Atsushi5, Katayama, Kazufumi1, Kudo, Chiho1, Kamisaki, Yoshinori1, Serhan, Charles N.2,3,4 cnserhan@zeus.bwh.harvard.edu |
| المصدر: |
FASEB Journal. Sep2006, Vol. 20 Issue 11, p1785-1792. 8p. 4 Diagrams, 4 Graphs. |
| مصطلحات موضوعية: |
*LEUKOTRIENES, *LIPOXINS, *STEM cells, *NEURAL stem cells, *INFLAMMATORY mediators |
| مستخلص: |
Leukotrienes (LTs) and lipoxins (LXs) are lipid mediators that play a key role in regulating acute inflammatory responses. Their roles in neural stem cell (NSC) functions are of interest. We showed here that LTB4 and LXA4 regulated proliferation and differentiation of murine NSCs that were isolated from embryo brains. Proliferation of NSCs was stimulated by LTB4 (3 to 100 nM) and blocked by receptor antagonist (IC50=2.7 µM). In contrast, LXA4, and its aspirin-triggered-15-epi-LXA4 stable analog attenuated growth of NSCs at as little as 1 nM. Both lipoxygenase (LOX) inhibitors and LTB4 receptor antagonists caused apoptosis and cell death. Gene chip analysis revealed that growth-related gene expressions such as epidermal growth factor (EGF) receptor, cyclin E, p27, and caspase 8 were tightly regulated by LTB4; LXA4 gave the opposite gene expressions. In addition to proliferation, LTB4 induced differentiation of NSCs into neurons as monitored by neurite outgrowth and MAP2 expression. These results indicate for the first time that LTB4 and LXA4 directly regulate proliferation and differentiation of NSCs, suggesting these new pathways may be useful in restoring stem cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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