دورية أكاديمية
Hepsin-mediated Processing of Uromodulin is Crucial for Salt-sensitivity and Thick Ascending Limb Homeostasis.
| العنوان: | Hepsin-mediated Processing of Uromodulin is Crucial for Salt-sensitivity and Thick Ascending Limb Homeostasis. |
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| المؤلفون: | Olinger, Eric, Lake, Jennifer, Sheehan, Susan, Schiano, Guglielmo, Takata, Tomoaki, Tokonami, Natsuko, Debaix, Huguette, Consolato, Francesco, Rampoldi, Luca, Korstanje, Ron, Devuyst, Olivier |
| المصدر: | Faculty Research 2019 |
| بيانات النشر: | The Mouseion at the JAXlibrary |
| سنة النشر: | 2019 |
| المجموعة: | The Jackson Laboratory: The Mouseion at the JAXlibrary |
| مصطلحات موضوعية: | JMG, Life Sciences, Medicine and Health Sciences |
| الوصف: | Uromodulin is a zona pellucida-type protein essentially produced in the thick ascending limb (TAL) of the mammalian kidney. It is the most abundant protein in normal urine. Defective uromodulin processing is associated with various kidney disorders. The luminal release and subsequent polymerization of uromodulin depend on its cleavage mediated by the serine protease hepsin. The biological relevance of a proper cleavage of uromodulin remains unknown. Here we combined in vivo testing on hepsin-deficient mice, ex vivo analyses on isolated tubules and in vitro studies on TAL cells to demonstrate that hepsin influence on uromodulin processing is an important modulator of salt transport via the sodium cotransporter NKCC2 in the TAL. At baseline, hepsin-deficient mice accumulate uromodulin, along with hyperactivated NKCC2, resulting in a positive sodium balance and a better adaptation to water deprivation. In conditions of high salt intake, defective uromodulin processing predisposes hepsin-deficient mice to a salt-wasting phenotype, with a decreased salt sensitivity. These modifications are associated with intracellular accumulation of uromodulin, endoplasmic reticulum-stress and signs of tubular damage. These studies expand the physiological role of hepsin and uromodulin and highlight the importance of hepsin-mediated processing of uromodulin for kidney tubule homeostasis and salt sensitivity. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://mouseion.jax.org/stfb2019/188Test; https://mouseion.jax.org/context/stfb2019/article/1183/viewcontent/12287.pdfTest |
| DOI: | 10.1038/s41598-019-48300-3 |
| الإتاحة: | https://doi.org/10.1038/s41598-019-48300-3Test https://mouseion.jax.org/stfb2019/188Test https://mouseion.jax.org/context/stfb2019/article/1183/viewcontent/12287.pdfTest |
| رقم الانضمام: | edsbas.6A8B06A5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-48300-3 |
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