دورية أكاديمية
Interactome maps of mouse gene regulatory domains reveal basic principles of transcriptional regulation.
العنوان: | Interactome maps of mouse gene regulatory domains reveal basic principles of transcriptional regulation. |
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المؤلفون: | Kieffer-Kwon, Kyong-Rim, Tang, Zhonghui, Mathe, Ewy, Qian, Jason, Sung, Myong-Hee, Li, Guoliang, Resch, Wolfgang, Baek, Songjoon, Pruett, Nathanael, Grøntved, Lars, Vian, Laura, Nelson, Steevenson, Zare, Hossein, Hakim, Ofir, Reyon, Deepak, Yamane, Arito, Nakahashi, Hirotaka, Kovalchuk, Alexander L, Zou, Jizhong, Joung, J Keith, Sartorelli, Vittorio, Wei, Chia-Lin, Ruan, Xiaoan, Hager, Gordon L, Ruan, Yijun, Casellas, Rafael |
المصدر: | Faculty Research 2013 |
بيانات النشر: | The Mouseion at the JAXlibrary |
سنة النشر: | 2013 |
المجموعة: | The Jackson Laboratory: The Mouseion at the JAXlibrary |
مصطلحات موضوعية: | Life Sciences, Medicine and Health Sciences |
الوصف: | A key finding of the ENCODE project is that the enhancer landscape of mammalian cells undergoes marked alterations during ontogeny. However, the nature and extent of these changes are unclear. As part of the NIH Mouse Regulome Project, we here combined DNaseI hypersensitivity, ChIP-seq, and ChIA-PET technologies to map the promoter-enhancer interactomes of pluripotent ES cells and differentiated B lymphocytes. We confirm that enhancer usage varies widely across tissues. Unexpectedly, we find that this feature extends to broadly transcribed genes, including Myc and Pim1 cell-cycle regulators, which associate with an entirely different set of enhancers in ES and B cells. By means of high-resolution CpG methylomes, genome editing, and digital footprinting, we show that these enhancers recruit lineage-determining factors. Furthermore, we demonstrate that the turning on and off of enhancers during development correlates with promoter activity. We propose that organisms rely on a dynamic enhancer landscape to control basic cellular functions in a tissue-specific manner. Cell 2013 Dec 19; 155(7):1507-20. |
نوع الوثيقة: | text |
اللغة: | unknown |
العلاقة: | https://mouseion.jax.org/stfb2013/191Test; http://dx.doi.org/10.1016/j.cell.2013.11.039Test |
DOI: | 10.1016/j.cell.2013.11.039 |
الإتاحة: | https://doi.org/10.1016/j.cell.2013.11.039Test https://mouseion.jax.org/stfb2013/191Test |
رقم الانضمام: | edsbas.4B024538 |
قاعدة البيانات: | BASE |
DOI: | 10.1016/j.cell.2013.11.039 |
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