التفاصيل البيبلوغرافية
| العنوان: |
Lipid-mediated protein functionalization of electrospun polycaprolactone fibers. |
| المؤلفون: |
Cohn, C., Leung, S. L., Crosby, J., Lafuente, B., Zha, Z., Teng, W., Downs, R., Wu, X. |
| المصدر: |
Express Polymer Letters; May2016, Vol. 10 Issue 5, p430-437, 8p, 1 Black and White Photograph, 2 Diagrams, 1 Graph |
| مصطلحات موضوعية: |
ELECTROSPINNING, POLYESTER fibers, POLYCAPROLACTONE, PROTEIN engineering, PLASMA materials processing, COATING processes |
| مستخلص: |
In this study, electrospun polycaprolactone (PCL) fibers are plasma-treated and chemically conjugated with cholesteryl succinyl silane (CSS). In addition to Raman spectroscopy, an immobilization study of DiO as a fluorescent probe of lipid membranes provides evidence supporting the CSS coating of plasma-treated PCL fibers. Further, anti-CD20 antibodies are used as a model protein to evaluate the potential of lipid-mediated protein immobilization as a mechanism to functionalize the CSS-PCL fiber scaffolds. Upon anti-CD20 functionalization, the CSS-PCL fiber scaffolds capture Granta-22 cells 2.4 times more than the PCL control does, although the two fiber scaffolds immobilize a comparable amount of anti-CD20. Taken together, results from the present study demonstrate that the CSS coating and CSS-mediated antibody immobilization offers an appealing strategy to functionalize electrospun synthetic polymer fibers and confer cell-specific functions on the fiber scaffolds, which can be mechanically robust but often lack biological functions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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