التفاصيل البيبلوغرافية
| العنوان: |
Lipidomic analysis of meibomian glands from type-1 diabetes mouse model and preliminary studies of potential mechanism. |
| المؤلفون: |
Wang, Huifeng1,2 (AUTHOR), Zhou, Qingjun2,3 (AUTHOR), Wan, Luqin1,2 (AUTHOR), Guo, Menglong4 (AUTHOR), Chen, Chen2 (AUTHOR), Xue, Junfa2 (AUTHOR), Yang, Lingling1,2,3 (AUTHOR) lingling6008@126.com, Xie, Lixin1,2,3 (AUTHOR) lixin_xie@hotmail.com |
| المصدر: |
Experimental Eye Research. Sep2021, Vol. 210, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*MEIBOMIAN glands, *LABORATORY mice, *DIABETES, *ANIMAL disease models, *ETIOLOGY of diabetes |
| مستخلص: |
Diabetes is a significant risk factor for meibomian gland dysfunction (MGD), but its mechanism is poorly understood. The main function of the meibomian glands (MGs) is to synthesize, store, and secrete lipids. In this study, we found that the amount of lipids in the meibomian acini in STZ-induced type 1 diabetic mice decreased, and the lipid droplets became larger and irregular. In all, 31 lipid subclasses were identified in the mouse MGs, which contained 1378 lipid species in total through lipidomics analysis based on LC-MS/MS. Diabetes caused a significant increase in the content of ceramides (Cer) in the MGs but a significant decrease in the ration of sphingomyelin to ceramides (SM/Cer). The quantity of meibocytes in diabetic mice was dramatically decreased, and the proliferation activity was alleviated, which may be associated with cell cycle arrest caused by diabetes-induced abnormal Cer metabolism in MGs. We found an increase in macrophage and neutrophils infiltration in the diabetic MGs, which may be related to the significant reduction of AcCa in diabetic MGs. Taken together, the results of the present study demonstrated that diabetes induced disruption of lipid homeostasis in MGs, which may mediate the decreased cell proliferation and increased inflammation caused by diabetes in MGs. • Diabetes induced disruption of lipid homeostasis in MGs. • Diabetes can lead to the meibomian gland dysfunction (MGD) in mice. • Cell proliferation and inflammation may contribute to the pathogenesis. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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