التفاصيل البيبلوغرافية
| العنوان: |
基于网络药理学的清爽膏治疗银屑病的作用机制研究. |
| العنوان البديل: |
Mechanism of Qingshuang Ointment in the Treatment of Psoriasis Based on Network Pharmacology. |
| المؤلفون: |
李 洋1 li_111126@126.com, 张林华2, 曾祖平3 zengzuping@bjzhongi.com |
| المصدر: |
Evaluation & Analysis of Drug-Use in Hospitals of China. 2022, Vol. 22 Issue 1, p10-19. 6p. |
| مصطلحات موضوعية: |
*VASCULAR endothelial growth factors, *CHINESE medicine, *CELLULAR signal transduction, *PROTEIN-protein interactions, *GENE ontology |
| مصطلحات جغرافية: |
KYOTO (Japan) |
| الملخص (بالإنجليزية): |
OBJECTIVE: To explore the effective material basis and mechanism of Qingshuang ointment in the treatment of psoriasis. METHODS: The active compounds and targets of three kinds of traditional Chinese medicine in Qingshuang ointment were searched by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, GeneCards database was used to retrieve the related targets of psoriasis, and Venn diagram was constructed to obtain the therapeutic targets of Qingshuang ointment in the treatment of psoriasis. Cytoscape 3. 7. 0 was used to perform the "drugs-compounds-targets" network. Protein-protein interaction network ( PPI) was constructed based on the common targets of Qingshuang ointment and psoriasis. Gene ontology (GO) functional enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed via DAVID v6. 8 database. RESULTS: Taking drug-like properties ≥0. 18 as criteria, 108 active compounds of Qingshuang ointment were screened, with 150 action targets. A total of 3 881 targets related to psoriasis were screened based on high correlation criteria, there were 82 common targets of drugs and diseases. PPI network was constructed, the most common targets were respectively IL-6, TP53, VEGFA, TNF, STAT3, PTGS2, CASP3, JUN and MAPK8. A total of 501 results were included in the GO enrichment analysis. The KEGG signal pathway enrichment showed that the common target was expressed in 98 signal pathways. CONCLUSIONS: This study indicates that Qingshuang ointment exerts its therapeutic effects on psoriasis through multiple components and targets, and the mechanism mainly focuses on the regulation of HIF-1 signaling pathway, PI3K-Akt signaling pathway, p53 signaling pathway, MAPK signaling pathway and vascular endothelial growth factor signaling pathway, which provides a scientific basis for the clinical application and experimental research of Qingshuang ointment. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的:探讨清爽膏治疗银屑病的药效物质基础及其作用机制。方法:通过网络药理学方法,借助于中药系统药理学数据库与分析平台检索清爽膏中3味中药的活性化合物和作用靶点,通过Gene Cards数据库检索银屑病的相关靶点,构建韦恩图,得到清爽膏治疗银屑病的作用靶点,应用Cytoscape 3.7.0软件构建"药物-化合物-靶点"网络图。针对清爽膏和银屑病的共同靶点,构建蛋白质-蛋白质相互作用（PPI）网络,进而通过DAVID v6.8数据库完成基因本体（GO）功能富集分析及京都基因与基因组百科全书（KEGG）通路富集分析。结果:以类药性≥0.18为标准,筛选出清爽膏的活性化合物108种,作用靶点150个;以高相关性为标准,筛选出与银屑病相关的靶点3 881个。药物与疾病的共同靶点有82个。构建PPI网络,相互作用关系最多的靶点为IL-6、TP53、VEGFA、TNF、STAT3、PTGS2、CASP3、JUN和MAPK8。GO功能富集分析包含501条结果,KEGG通路富集分析得到98条信号通路。结论:本研究阐明清爽膏是通过多成分、多靶点发挥对银屑病的治疗作用,且作用机制主要集中在调节HIF-1 信号通路、PI3K-Akt 信号通路、p53信号通路、MAPK 信号通路和血管内皮生长因子信号通路, 为清爽膏的临床应用和实验研究提供了科学依据。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
