Canine Pancreatic Islet Transplantation: A Comparison of Two Isolation Techniques
| العنوان: | Canine Pancreatic Islet Transplantation: A Comparison of Two Isolation Techniques |
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| المؤلفون: | J. A. Vand Der Vliet, Dixon B. Kaufman, D. E. R. Sutherland, Meloche Rm, L. G. Van Der Hem, M.Jane Field |
| المصدر: | European Surgical Research. 22:1-7 |
| بيانات النشر: | S. Karger AG, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Blood Glucose, Male, endocrine system, medicine.medical_specialty, Pathology, Tissue and Organ Procurement, Islets of Langerhans Transplantation, Portal vein, Transplantation, Autologous, Islets of Langerhans, Dogs, Internal medicine, medicine, Isolation techniques, Animals, Insulin, Postoperative Period, geography, geography.geographical_feature_category, business.industry, Fasting, Glucose Tolerance Test, Islet, Transplantation, medicine.anatomical_structure, Endocrinology, Evaluation Studies as Topic, Collagenase, Female, Surgery, Pancreatic islet transplantation, Pancreas, business, Perfusion, medicine.drug |
| الوصف: | A new method (type B) for isolation of canine islets was developed, utilizing intraductal collagenase perfusion, stationary digestion, filtration and tissue separation by means of a dextran density gradient. The results of this technique were compared with those of a previously established method (type A). Islets were autotransplanted either via the splenic or the portal vein. Lasting normoglycemia was obtained in 5/8 intrasplenic type B transplants (63%), 6/8 intrahepatic type B transplants (75%), 5/6 intrasplenic type A transplants (83%) and 0/3 intrahepatic type A transplants. No difference was found in metabolic parameters 2 weeks after successful transplantation following type A or B islet isolation. Islet yield was higher with type A and purification better with type B isolation. Both techniques are relatively simple and inexpensive. Because of its higher purification rate and the success of intrahepatic islet transplantation method B has replaced method A as the routine procedure in our laboratory. |
| تدمد: | 1421-9921 0014-312X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0d3b13cbd8caef583b57228ccc16361Test https://doi.org/10.1159/000129075Test |
| رقم الانضمام: | edsair.doi.dedup.....a0d3b13cbd8caef583b57228ccc16361 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219921 0014312X |
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