Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model
| العنوان: | Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model |
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| المؤلفون: | Alex Nasare, Luis Garcia Alonso, Heitor Franco de Andrade, Roberto Tedesco, Andrés Jimenez Galisteo, Priscila Cardoso Cristovam, José Álvaro Pereira Gomes, Helene Santos Barbosa, Érik V. Guimarães, Marcos A. Cenedese |
| المصدر: | European Journal of Microbiology & Immunology |
| بيانات النشر: | Akadémiai Kiadó, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | parasitic ocular infections, Pathology, medicine.medical_specialty, retina, experimental models, chemistry.chemical_compound, Heat shock protein, Gene expression, medicine, Gene, Retina, Retinal pigment epithelium, biology, ocular toxoplasmosis, Toxoplasma gondii, Retinal, embryo development, biology.organism_classification, medicine.disease, Toxoplasmosis, regulation of gene expression in development, medicine.anatomical_structure, chemistry, Immunology, Original Article, sense organs |
| الوصف: | HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos' eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny. |
| اللغة: | English |
| تدمد: | 2062-8633 2062-509X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1db49db956e69b5b041b38f6d2c5dd8bTest http://europepmc.org/articles/PMC4681359Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1db49db956e69b5b041b38f6d2c5dd8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20628633 2062509X |
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