The expression of cytosolic phospholipase A2and biosynthesis of leukotriene B4in acute myeloid leukemia cells
| العنوان: | The expression of cytosolic phospholipase A2and biosynthesis of leukotriene B4in acute myeloid leukemia cells |
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| المؤلفون: | Stina Feltenmark, Gudmundur Runarsson, Magnus Björkholm, Pontus Forsell, Hans-Erik Claesson, Jan Sjöberg |
| المصدر: | European Journal of Haematology. 79:468-476 |
| بيانات النشر: | Wiley, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Adult, Male, Leukotriene B4, CD34, Phospholipases A2, Cytosolic, Antigens, CD34, Biology, Models, Biological, chemistry.chemical_compound, Phospholipase A2, Granulocyte Colony-Stimulating Factor, Humans, Calcimycin, Aged, Phospholipase A, Arachidonate 5-Lipoxygenase, Ionophores, Gene Expression Regulation, Leukemic, Myeloid leukemia, Hematology, General Medicine, Middle Aged, Molecular biology, Leukemia, Myeloid, Acute, Biochemistry, chemistry, Arachidonate 5-lipoxygenase, biology.protein, Female, Arachidonic acid, Myelopoiesis |
| الوصف: | Leukotrienes (LT) exert stimulatory effects on myelopoiesis, beside their inflammatory and immunomodulating effects. Here, we have studied the expression and activity of the enzymes involved in the synthesis of leukotriene B 4 (LTB 4 ) in acute myeloid leukemia (AML) cells (16 clones) and G-CSF mobilized peripheral blood CD34 + cells. CD34 + cells from patients with non-myeloid malignancies expressed cytosolic phospholipase A 2 (cPLA 2 ), 5-lipoxygenase activating protein (FLAP), and leukotriene A 4 (LTA 4 ) hydrolase but not 5-lipoxygenase (5-LO). The enzyme cPLA2 was abundantly expressed in AML cells and the activity of the enzyme was high in certain AML clones. The expression of 5-LO, FLAP, and LTA 4 hydrolase in AML clones was in general lower than in healthy donor polymorphonuclear leukocytes (PMNL). The calcium ionophore A23187-induced release of [ 14 C] arachidonic acid (AA) in AML cells was low, compared with PMNL, and did not correlate with the expression of cPLA2 protein. Biosynthesis of LTB 4 , upon calcium ionophore A23187 activation, was only observed in five of the investigated AML clones and only three of the most differentiated clones produced similar amounts of LTB 4 as PMNL. The capacity of various cell clones to produce LTs could neither be explained by the difference in [1- 14 C] AA release nor 5-LO expression. Taken together, these results indicate that LT synthesis is under development during early myelopoiesis and the capacity to produce LTs is gained upon maturation. High expression of cPLA2 in AML suggests a putative role of this enzyme in the pathophysiology of this disease. |
| تدمد: | 1600-0609 0902-4441 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89284a46a68badf8b6cfeee70d57ca1fTest https://doi.org/10.1111/j.1600-0609.2007.00967.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....89284a46a68badf8b6cfeee70d57ca1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000609 09024441 |
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