Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori
| العنوان: | Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori |
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| المؤلفون: | Francesco Perri, Maurizio Zagari R, Gerardo Nardone, Maria Imeneo, Paolo Usai, Franco Bazzoli, Crotta S, Marasco A, Francesco Luzza, Enzo Ierardi, Sanna G, Virgilio C, Santino Marchi |
| المصدر: | Scopus-Elsevier Europe PubMed Central |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Saliva, Adolescent, Sensitivity and Specificity, Gastroenterology, Helicobacter Infections, Serology, Immunoenzyme Techniques, Predictive Value of Tests, Saliva testing, Positive predicative value, Internal medicine, medicine, Humans, Serologic Tests, Prospective Studies, Aged, Aged, 80 and over, Breath test, Helicobacter pylori, Hepatology, medicine.diagnostic_test, biology, Receiver operating characteristic, business.industry, Middle Aged, biology.organism_classification, ROC Curve, Immunoglobulin G, Immunoassay, Immunology, Female, business |
| الوصف: | Objective To evaluate the usefulness of a commercial serological enzyme immunoassay (EIA) for detecting salivary immunoglobulin (Ig) G to Helicobacter pylori. Design Prospective, multicentre study. Setting The study was conducted at 11 gastroenterology hospital centres throughout Italy. Participants and interventions Two hundred and thirteen dyspeptic patients underwent gastroscopy with antral biopsies. At each centre, two of the following three tests for H. pylori diagnosis were performed: urease quick test, histology, and 13 C-urea breath test. Samples of unstimulated saliva and venous blood were collected from each patient. Salivary and serum H. pylori IgG were determined with the EIA Helori-test IgG (Eurospital, Trieste, Italy). Main outcome measures Sensitivity, specificity, positive and negative predictive values, and accuracy of the salivary and serum ElAs. Results By using a receiver operating characteristic curve, salivary EIA yielded 81% (95% confidence interval 73-87%) sensitivity, 73% (62-83%) specificity, 84% (76-90%) positive predictive value, 69% (58-79%) negative predictive value, and 78% (72-84%) accuracy. At the cut-off suggested by the manufacturer, serum EIA had 90% (84-95%) sensitivity, 78% (67-86%) specificity, 88% (81 -93%) positive predictive value, 82% (71-90%) negative predictive value, and 86% (81 -91%) accuracy. Conclusion In this large multicentre study, detection of salivary H. pylori IgG with a commercial serological EIA was a fairly accurate diagnostic method. Data confirm that saliva testing does not compare favourably with serology in the assessment of H. pylori status. |
| تدمد: | 0954-691X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74960684e69d7d086f22fa79716db1b1Test https://doi.org/10.1097/00042737-200012100-00008Test |
| رقم الانضمام: | edsair.doi.dedup.....74960684e69d7d086f22fa79716db1b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0954691X |
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