Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term
| العنوان: | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
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| المؤلفون: | Ina Stuen, Lene Østby, Arne Reimers, Eirik Sundby |
| المصدر: | European Journal of Drug Metabolism and Pharmacokinetics |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | medicine.medical_specialty, UGT1A4, Glucuronosyltransferase, Clinical chemistry, Placenta, Blotting, Western, Biology, Lamotrigine, Isozyme, Gene Expression Regulation, Enzymologic, Western blot, Pregnancy, Microsomes, Internal medicine, medicine, Humans, Pharmacology (medical), reproductive and urinary physiology, Pharmacology, Original Paper, Epilepsy, medicine.diagnostic_test, Triazines, medicine.disease, Pregnancy Complications, Endocrinology, medicine.anatomical_structure, Case-Control Studies, embryonic structures, Microsome, biology.protein, Anticonvulsants, Female, UGT |
| الوصف: | The placenta contains a large variety of metabolizing enzymes, among them UDP-glucuronosyltransferase (UGT). Several UGT2B isozymes have so far been detected in human placenta, but little is known on placental expression of UGT1A isozymes. The antiepileptic drug lamotrigine (LTG) is a UGT1A4-substrate, and its serum concentration falls by over 50% during pregnancy, leading to impaired seizure control. The placenta may be involved in this. Microsomes from term placentas of 4 LTG-users and 10 healthy control subjects were prepared. Western blot analysis detected UGT1A proteins in all placentas. The presence of UGT1A4 in placenta from LTG users was confirmed with UGT1A4 commercial standard and a specific UGT1A4 primary antibody. Since LTG is primarily metabolized by UGT1A4 and this isozyme is shown to be present in placenta at term, it may be hypothesized that the placenta is involved in the fall of LTG serum concentrations during pregnancy. |
| تدمد: | 2107-0180 0378-7966 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aed616be8c95f1f1abca6231b3ccba3aTest https://doi.org/10.1007/s13318-010-0021-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aed616be8c95f1f1abca6231b3ccba3a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21070180 03787966 |
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