Clinical evaluation of rapid point-of-care antigen tests for diagnosis of SARS-CoV-2 infection
| العنوان: | Clinical evaluation of rapid point-of-care antigen tests for diagnosis of SARS-CoV-2 infection |
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| المؤلفون: | David S. Y. Ong, Stijn J. de Man, Johannes G.M. Koeleman, Henk Brand |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases |
| بيانات النشر: | Springer Berlin Heidelberg, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Population, Sensitivity and Specificity, COVID-19 Serological Testing, 03 medical and health sciences, 0302 clinical medicine, Medical microbiology, Antigen, Lateral flow immunoassay, Internal medicine, medicine, Humans, 030212 general & internal medicine, Prospective Studies, education, Antigens, Viral, Point of care, education.field_of_study, business.industry, SARS-CoV-2, COVID-19, General Medicine, Gold standard (test), 030104 developmental biology, Infectious Diseases, Rapid antigen test, Point-of-Care Testing, Population study, Original Article, business, Viral load, POC test |
| الوصف: | The RT-qPCR in respiratory specimens is the gold standard for diagnosing acute COVID-19 infections. However, this test takes considerable time before test results become available, thereby delaying patients from being diagnosed, treated, and isolated immediately. Rapid antigen tests could overcome this problem. In the first study, clinical performances of five rapid antigen tests were compared to RT-qPCR in upper respiratory specimens from 40 patients with positive and 40 with negative RTq-PCR results. In the second study, the rapid antigen test with one of the best test characteristics (Romed) was evaluated in a large prospective collection of upper respiratory specimens from 900 different COVID-19-suspected patients (300 emergency room patients, 300 nursing home patients, and 300 health care workers). Test specificities ranged from 87.5 to 100.0%, and test sensitivities from 55.0 to 80.0%. The clinical specificity of the Romed test was 99.8% (95% CI 98.9–100). Overall clinical sensitivity in the study population was 73.3% (95% CI 67.9–78.2), whereas sensitivity in the different patient groups varied from 65.3 to 86.7%. Sensitivity was 83.0 to 86.7% in patients with short duration of symptoms. In a population with a COVID-19 prevalence of 1%, the negative predictive value in all patients was 99.7%. There is a large variability in diagnostic performance between rapid antigen tests. The Romed rapid antigen test showed a good clinical performance in patients with high viral loads (RT-qPCR cycle threshold ≤30), which makes this antigen test suitable for rapid identification of COVID-19-infected health care workers and patients. |
| اللغة: | English |
| تدمد: | 1435-4373 0934-9723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf6babd4e202f95fb00743c5d6d469c4Test http://europepmc.org/articles/PMC8140309Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bf6babd4e202f95fb00743c5d6d469c4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14354373 09349723 |
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