التفاصيل البيبلوغرافية
| العنوان: |
Association of CK19 mRNA detection of occult cancer cells in mediastinal lymph nodes in non-small cell lung carcinoma and high risk of early recurrence |
| المؤلفون: |
Pimpec-Barthes, Françoise Le1 francoise.le-pimpec-barthes@hop.egp.ap-hop-paris.fr, Danel, Claire2, Lacave, Roger3, Ricci, Sylvie3, Bry, Xavier4, Lancelin, Frédérique3, Leber, Cécile3, Milleron, Bernard5, Fleury-Feith, Jocelyne3, Riquet, Marc1, Bernaudin, Jean-François3 |
| المصدر: |
European Journal of Cancer. Jan2005, Vol. 41 Issue 2, p306-312. 7p. |
| مصطلحات موضوعية: |
*CANCER cells, *CANCER invasiveness, *DNA polymerases, *TOMOGRAPHY, *IMMUNOHISTOCHEMISTRY |
| مستخلص: |
Abstract: This study was designed to screen occult cancer cells by CK19 mRNA detection using reverse transcriptase-polymerase chain reaction (RT-PCR) in mediastinal lymph nodes stations (MLNS) in non-small cell lung carcinoma (NSCLC). In 49 NSCLC patients free of mediastinal adenopathy on computed tomograph, 254 MLNS were evaluated by histopathology, immunohistochemistry (IHC) and RT-PCR. Of 225 non-tumoral MLNS on histopathology, 32 (14.2%) were positive by RT-PCR. IHC did not provide significant additional results. Seventeen patients were without mediastinal tumoral extension on histopathology and RT-PCR (Group 1), 16 were upgraded by RT-PCR (Group 2) and 16 pN2 on histopathology (Group 3). The two-year cancer-related death survival in Groups 1 (100%) and 2 (64.5%) was significantly different (P=0.04). The relative risk of recurrence in Group 2 compared with Group 1, evaluated by the Cox model multivariate analysis, was 5.61 (P=0.02). In conclusion, CK19 mRNA detected by RT-PCR in MLNS was significantly associated with an increased risk of rapid recurrence. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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