Identification of European commercial cockles (Cerastoderma edule and C. glaucum) by species-specific PCR amplification of the ribosomal DNA ITS region
| العنوان: | Identification of European commercial cockles (Cerastoderma edule and C. glaucum) by species-specific PCR amplification of the ribosomal DNA ITS region |
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| المؤلفون: | Josefina Méndez, Ruth Freire, Alberto Arias, Ana Insua |
| المصدر: | European Food Research and Technology. 232:83-86 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cerastoderma edule, General Chemistry, Biology, biology.organism_classification, Biochemistry, Molecular biology, Industrial and Manufacturing Engineering, law.invention, law, Identification (biology), Multiplex, Cockle, Internal transcribed spacer, Primer (molecular biology), Ribosomal DNA, Polymerase chain reaction, Food Science, Biotechnology |
| الوصف: | We present a novel one-step PCR method for the identification of Cerastoderma edule and C. glaucum. Sequence differences found in the internal transcribed spacer region (ITS) of the ribosomal DNA of the two cockles allowed us to design two species-specific reverse primers. These two primers were used in a multiplex reaction, together with a forward universal primer to amplify specific fragments with different lengths in each cockle (190 and 470 bp in C. edule and C. glaucum, respectively). The successful and specific amplifications obtained for two natural populations in each species as well as in canned products lend support to the usefulness of these markers. |
| تدمد: | 1438-2385 1438-2377 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::08001c578b79fb54cd2521cf6e8e9e28Test https://doi.org/10.1007/s00217-010-1369-5Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........08001c578b79fb54cd2521cf6e8e9e28 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14382385 14382377 |
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