التفاصيل البيبلوغرافية
| العنوان: |
Isolation, identification, and molecular docking analysis of novel ACE inhibitory peptides from Spirulina platensis. |
| المؤلفون: |
Zhang, Nan1 (AUTHOR), Li, Fuqiang1 (AUTHOR), Zhang, Tingxin1 (AUTHOR), Li, Chun-Yang2 (AUTHOR), Zhu, Liping1 (AUTHOR), Yan, Shigan1 (AUTHOR) yanshigan@126.com |
| المصدر: |
European Food Research & Technology. Apr2022, Vol. 248 Issue 4, p1107-1115. 9p. |
| مصطلحات موضوعية: |
*ANGIOTENSIN I, *SPIRULINA platensis, *MOLECULAR docking, *MASS spectrometry, *PEPTIDES, *REGULATION of blood pressure |
| مستخلص: |
Hypertension has emerged as a serious global health issue and piqued the interest of academics all over the world. Angiotensin-I converting enzyme (ACE, EC 3.4.15.1) plays a crucial role in blood pressure regulation. Due to their safety and mildness compared with synthetic drugs, natural bioactive inhibitory peptides against ACE generated from various protein sources cause an upsurge of research and development. Spirulina platensis is a cyanobacterium widely distributed in aquaculture and an ideal protein source for bioactive peptide production, owing to its remarkable protein content and nutritive value. In this study, novel ACE inhibitory peptides from Spirulina platensis protein hydrolysates digested by commercial thermolysin were isolated by membrane filtration and size exclusion-high-performance liquid chromatography (SEC-HPLC) in sequence, and identified by mass spectrum analysis and ACE inhibitory activity assay. The results showed that two novel ACE inhibitory peptides were obtained, and their amino acid sequences were Val–Thr–Tyr (VTY) and Leu–Gly–Val–Pro (LGVP), with IC50 values of 23.39 µM, and 45.76 µM, respectively. Molecular docking analysis indicated that the VTY was mainly bound in the S1 and S2 active pockets of ACE via hydrogen bonds, while LGVP only partially interacted with the S1 pocket. Our findings provide novel insights for the development of ACE inhibitors and the high-value utilization of Spirulina platensis. [ABSTRACT FROM AUTHOR] |
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