Vehicle-to-grid presents a mechanism to meet the key requirements of an electric power system, using electric vehicles (EVs) when they are parked. The most economic ancillary service is that of frequency regulation, which imposes some constraints regarding the period and duration of time the vehicles have to be connected to the grid. The majority of research explores the profitability of the aggregator, while the perspective of the EV fleet owner, in terms of their need for usage of their fleet, remains neglected. In this paper, the optimal allocation of available vehicles on a day-ahead basis using queuing theory and fuzzy multi-criteria methodology has been determined. The proposed methodology is illustrated on the daily scheduling of EVs in an electricity distribution company.