دورية أكاديمية
Dietary inclusion of plant ingredients induces epigenetic changes in the intestine of zebrafish
العنوان: | Dietary inclusion of plant ingredients induces epigenetic changes in the intestine of zebrafish |
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المؤلفون: | Anusha Dhanasiri, Xianquan Chen, Dalia Dahle, Prabhugouda Siriyappagouder, Christiane K. Fæste, Jorge M. O. Fernandes |
المصدر: | Epigenetics, Vol 15, Iss 10, Pp 1035-1051 (2020) |
بيانات النشر: | Taylor & Francis Group, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Genetics |
مصطلحات موضوعية: | epigenetics, dna methylation, plant-based proteins, intestine, inflammation, pea protein concentrate, soy protein concentrate, wheat gluten, Genetics, QH426-470 |
الوصف: | Epigenetic modifications, such as DNA methylation, can be regulated by nutrition and dietary factors. There has been a large increase in the use of sustainable plant-based protein sources in fish feed due to limitations of fishmeal resources, which are needed to sustain a rapidly growing aquaculture industry. With this major transition from marine ingredients to plant-based diets, fish are abruptly introduced to changes in dietary composition and exposed to a variety of phytochemicals, some of which known to cause epigenetic changes in mammals. However, the effect of plant ingredients on the epigenome of fish is barely understood. In the present study, the nutriepigenomic effects of the addition of pea, soy, and wheat gluten protein concentrate to aquafeeds were investigated using zebrafish as a model. A genome-wide analysis of DNA methylation patterns was performed by reduced representation bisulphite sequencing to examine global epigenetic alterations in the mid intestine after a 42-day feeding trial. We found that inclusion of 30% of wheat gluten, pea and soy protein concentrate in the diet induced epigenetic changes in the mid intestine of zebrafish. A large number of genes and intergenic regions were differentially methylated with plant-based diets. The genes concerned were related to immunity, NF‐κB system, ubiquitin-proteasome pathway, MAPK pathway, and the antioxidant defence system. Epigenetic regulation of several biological processes, including neurogenesis, cell adhesion, response to stress and immunity was also observed. Ultimately, the observed epigenetic changes may enable zebrafish to rapidly regulate inflammation and maintain intestinal homoeostasis when fed plant protein–based diets. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1559-2294 1559-2308 15592294 |
العلاقة: | https://doaj.org/toc/1559-2294Test; https://doaj.org/toc/1559-2308Test |
DOI: | 10.1080/15592294.2020.1747777 |
الوصول الحر: | https://doaj.org/article/bf5bfff11f054f97bcadff41f4baf588Test |
رقم الانضمام: | edsdoj.bf5bfff11f054f97bcadff41f4baf588 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15592294 15592308 |
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DOI: | 10.1080/15592294.2020.1747777 |