التفاصيل البيبلوغرافية
| العنوان: |
Whole-cell bioconversion for producing thymoquinone by engineered Saccharomyces cerevisiae. |
| المؤلفون: |
Kim, Eunjee1 (AUTHOR), Kim, Minsun1 (AUTHOR), Oh, Min-Kyu1 (AUTHOR) mkoh@korea.ac.kr |
| المصدر: |
Enzyme & Microbial Technology. Aug2024, Vol. 178, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*SACCHAROMYCES cerevisiae, *BIOCONVERSION, *CYTOCHROME P-450, *MICROBIAL cells, *ENDOPLASMIC reticulum, *OREGANO, *BLACK cumin |
| مستخلص: |
Thymoquinone, extracted from the black seeds of Nigella sativa , is a natural substance with highly beneficial effects against various human diseases. In this study, we aimed to construct a Saccharomyces cerevisiae strain that, produce thymoquinone from thymol, a relatively inexpensive substrate. To achieve this, cytochrome P450 from Origanum vulgare was expressed in S. cerevisiae for the bioconversion of thymol to thymoquinone, with the co-expression of cytochrome P450 reductase (CPR) from Arabidopsis thaliana , ATR1. Additionally, flexible linkers were used to connect these two enzymes. Furthermore, modifications were performed to expand the endoplasmic reticulum (ER) space, leading to increased thymoquinone production. After integrating the genes into the chromosome and optimizing the media components, a significant improvement in the thymol-to-thymoquinone conversion rate and yield were achieved. This study represents a possibility of the production of thymoquinone, a bioactive ingredient of a plant, using an engineered microbial cell. • Thymoquinone is a natural substance from a plant black seed with highly beneficial effects. • A cytochrome P450 for the bioconversion of thymol to thymoquinone was expressed in S. cerevisiae. • With the co-expression with ATR1 and expansion of ER, thymoquinone production was increased. • The production of thymoquinone in an engineered microbial cell was demonstrated for the first time. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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