التفاصيل البيبلوغرافية
| العنوان: |
Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters. |
| المؤلفون: |
ZHEN ZHANG1, JING-FU LIU1 jfliu@rcees.ac.cn, BING SHAO2, GUI-BIN JIANG1 |
| المصدر: |
Environmental Science & Technology. 2/1/2010, Vol. 44 Issue 3, p1030-1035. 6p. |
| مصطلحات موضوعية: |
ENVIRONMENTAL forensics, WATER analysis, SULFAMETHAZINE, SULFAMETHOXAZOLE, SULFADIAZINE, WATER sampling, RUNOFF, SEWAGE, ENZYME-linked immunosorbent assay, LIQUID chromatography, TANDEM mass spectrometry |
| مستخلص: |
Using monoclonal antibodies labeled with Eu3+ chelates, time-resolved fluoroimmunoassay (TRFIA) methods were developed for the determination of trace sulfamethazine (SMZ), sulfa-methoxazole (SMX), and sulfadiazine (SDZ) in environmental waters. Under the optimized conditions, the developed methods offered (i) low detection limits (9.8 ng/L SMZ, 6.1 ng/L SMX, and 5.4 ng/L SDZ, based on 90% inhibition) which were about 1 order of magnitude lower than that of the enzyme-linked immunosorbent assay (ELISA), (ii) high selectivity with no cross-reactivity (<0.05%) to similarly structured sulfonamides; (iii) high tolerance to variation of the sample pH (6.0-9.0) and salinity (0-100 mM), as well as the presence of humic acid (0-100 mg/L DOC) and heavy metals (0-1 mg/L concentration each of Cu2+, Cd2+, Hg2+, Pb2+ and As(V)) in the samples, and (iv) direct determination with low cost, high sample throughput, and low sample consumption (50-100 μL). The proposed TRFIA procedures were applied to determine sulfonamides in a variety of surface water and wastewater samples without sample pretreatment other than filtration. The satisfactory recoveries (64-127%) and reproducibilities (CV = 0.2-16%) achieved, as well as the good agreement with those given by liquid chromatography—tandem mass spectroscopy and ELISA methods, demonstrated the applicability of the proposed TRFIA methods for routine screening/quantification of sulfonamides in environmental waters. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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