Placental biomarkers of phthalate effects on mRNA transcription: application in epidemiologic research
| العنوان: | Placental biomarkers of phthalate effects on mRNA transcription: application in epidemiologic research |
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| المؤلفون: | Russ Hauser, Paige L. Williams, Harshwardhan M. Thaker, Heather H. Nelson, Robert F. Herrick, Jennifer J. Adibi, Robin M. Whyatt, Hari K. Bhat |
| المصدر: | Environmental Health, Vol 8, Iss 1, p 20 (2009) Environmental Health |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Candidate gene, Transcription, Genetic, Placenta, Health, Toxicology and Mutagenesis, Phthalic Acids, Gene Expression, Peroxisome proliferator-activated receptor, Biology, Andrology, Cyclophilins, lcsh:RC963-969, 03 medical and health sciences, Aromatase, 0302 clinical medicine, Gene expression, RNA, Ribosomal, 18S, medicine, Humans, RNA, Messenger, Gene, 030304 developmental biology, Regulation of gene expression, chemistry.chemical_classification, 0303 health sciences, 030219 obstetrics & reproductive medicine, Reverse Transcriptase Polymerase Chain Reaction, lcsh:Public aspects of medicine, Methodology, Public Health, Environmental and Occupational Health, Trophoblast, lcsh:RA1-1270, 3. Good health, PPAR gamma, medicine.anatomical_structure, Gene Expression Regulation, chemistry, lcsh:Industrial medicine. Industrial hygiene, Female, Sample collection, Biomarkers |
| الوصف: | Background CYP19 and PPARγ are two genes expressed in the placental trophoblast that are important to placental function and are disrupted by phthalate exposure in other cell types. Measurement of the mRNA of these two genes in human placental tissue by quantitative real-time polymerase chain reaction (qPCR) offers a source of potential biomarkers for use in epidemiologic research. We report on methodologic challenges to be considered in study design. Methods We anonymously collected 10 full-term placentas and, for each, sampled placental villi at 12 sites in the chorionic plate representing the inner (closer to the cord insertion site) and outer regions. Each sample was analyzed for the expression of two candidate genes, aromatase (CYP19) and peroxisome proliferator activated receptor protein gamma (PPARγ) and three potential internal controls: cyclophilin (CYC), 18S rRNA (18S), and total RNA. Between and within placenta variability was estimated using variance component analysis. Associations of expression levels with sampling characteristics were estimated using mixed effects models. Results We identified large within-placenta variability in both transcripts (>90% of total variance) that was minimized to Conclusion qPCR-derived biomarkers of placental CYP19 and PPARγ gene expression show high within-placental variability. Sampling scheme, selection of an appropriate internal control and the timing of sample collection relative to delivery can be optimized to minimize within-placenta and other sources of underlying, non-etiologic variability. |
| تدمد: | 1476-069X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf805a03662813906207736e04ec6cecTest https://doi.org/10.1186/1476-069x-8-20Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bf805a03662813906207736e04ec6cec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1476069X |
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