التفاصيل البيبلوغرافية
| العنوان: |
Measurement of estrogenic activity in sediments from Haihe and Dagu River, China |
| المؤلفون: |
Song, Maoyong1,2, Xu, Yan2, Jiang, Qinting2, Lam, Paul K.S.2, O'Toole, Desmond K.2, Giesy, John P.2,3, Jiang, Guibin1 gbjiang@mail.recees.ac.cn |
| المصدر: |
Environment International. Jul2006, Vol. 32 Issue 5, p676-681. 6p. |
| مصطلحات موضوعية: |
*ESTROGEN, *BIOLOGICAL assay, *STEROID hormones |
| مصطلحات جغرافية: |
CHINA |
| مستخلص: |
Abstract: Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g−1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3>Fraction 2>Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2>E1>NP>OP>BPA. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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