التفاصيل البيبلوغرافية
| العنوان: |
Detection of soluble biomarkers of pancreatic cancer in endoscopic ultrasound-guided fine-needle aspiration samples. |
| المؤلفون: |
Souche, Régis, Tosato, Guillaume, Rivière, Benjamin, Valats, Jean-Christophe, Debourdeau, Antoine, Flori, Nicolas, Pourquier, Didier, Fabre, Jean-Michel, Assenat, Eric, Colinge, Jacques, Turtoi, Andrei |
| المصدر: |
Endoscopy; May2022, Vol. 54 Issue 5, p503-508, 6p |
| مصطلحات موضوعية: |
PANCREATIC tumors, RESEARCH, CLINICAL trials, RESEARCH methodology, RETROSPECTIVE studies, EVALUATION research, DUCTAL carcinoma, PROTEOMICS, COMPARATIVE studies, RESEARCH funding, NEEDLE biopsy |
| مستخلص: |
Background: Biomarkers are urgently needed for pancreatic ductal adenocarcinoma (PDAC). Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is the cornerstone for diagnosing PDAC. We developed a method for discovery of PDAC biomarkers using the discarded EUS-FNA liquid.Methods: This retrospective study included 58 patients with suspected pancreatic lesions who underwent EUS-FNA. Protein extracts from EUS-FNA liquid were analyzed by mass spectrometry. Proteomic and clinical data were modeled by supervised statistical learning to identify protein markers and clinical variables that distinguish PDAC.Results: Statistical modeling revealed a protein signature for PDAC screening that achieved high sensitivity and specificity (0.92, 95 % confidence interval [CI] 0.79-0.98, and 0.85, 95 %CI 0.67-0.93, respectively). We also developed a protein signature score (PSS) to guide PDAC diagnosis. In combination with patient age, the PSS achieved 100 % certainty in correctly identifying PDAC patients > 54 years. In addition, 3 /4 inconclusive EUS-FNA biopsies were correctly identified using PSS.Conclusions: EUS-FNA-derived fluid is a rich source of PDAC proteins with biomarker potential. The PSS requires further validation and verification of the feasibility of measuring these proteins in patient sera. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
