Nur77 gene expression levels were involved in different ACTH-secretion autonomy between Cushing’s disease and subclinical Cushing’s disease
| العنوان: | Nur77 gene expression levels were involved in different ACTH-secretion autonomy between Cushing’s disease and subclinical Cushing’s disease |
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| المؤلفون: | Iichiro Shimomura, Eiichi Morii, Toshimitsu Hamasaki, Yukiko Tabuchi, Yugo Miyata, Youichi Saitoh, Atsunori Fukuhara, Michio Otsuki, Satoru Oshino, Kosuke Mukai, Tetsuhiro Kitamura, Toshiharu Onodera |
| المصدر: | Endocrine Journal. 63:545-554 |
| بيانات النشر: | Japan Endocrine Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adenoma, Adult, Male, 0301 basic medicine, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Gene Expression, Prohormone convertase, 030209 endocrinology & metabolism, Adrenocorticotropic hormone, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Glucocorticoid receptor, Adrenocorticotropic Hormone, Internal medicine, Nuclear Receptor Subfamily 4, Group A, Member 1, medicine, Humans, Pituitary ACTH Hypersecretion, Aged, Feedback, Physiological, Secretory Pathway, business.industry, Pituitary ACTH hypersecretion, Pituitary tumors, Cushing's disease, Middle Aged, medicine.disease, ACTH-Secreting Pituitary Adenoma, 030104 developmental biology, Hormone receptor, Case-Control Studies, Asymptomatic Diseases, Female, business, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Cushing's disease (CD) and subclinical Cushing's disease (subCD) are both diseases caused by adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas. However, ACTH autonomy in subCD is weaker than in CD and there are no Cushingoid features in subCD. The differences of molecular mechanisms in ACTH autonomy between CD and subCD have not yet been reported. Therefore, we aimed to investigate the differences in molecular mechanisms of ACTH-secretion autonomy between CD and subCD. The study included 23 patients [7 CD, 6 subCD, and 10 non-functioning pituitary tumors (NFTs)] who underwent transsphenoidal surgery at the Osaka University Hospital between December 2009 and October 2013. Using quantitative real-time PCR, various ACTH-related gene expressions in tumor tissues from CD, subCD, and NFT were measured such as pro-opiomelanocortin (POMC), POMC transcription factor (Tpit, Pitx1, NeuroD1, and Nur77), POMC peptide processing enzymes (prohormone convertase: PC1/3 and PC2), and ACTH secretion-related factors (corticotropin-releasing hormone receptor 1: CRHR1 and glucocorticoid receptor α: GRα). Only Nur77 mRNA levels were significantly higher in CD than in subCD. Furthermore, we stained 6 CD and 6 subCD with anti-Nur77 antibody. All tumor samples from CD had Nur77 protein positive cells. On the other hand, Nur77 protein was expressed in only one tumor sample from subCD. This sample showed high expression of Nur77 mRNA. Nur77 is an important to regulate POMC transcription and negative-feedback by glucocorticoids. Nur77 gene expression levels might involve different autonomy of ACTH production between CD and subCD. |
| تدمد: | 1348-4540 0918-8959 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dae424ee0c191af5e9ac2e570560b568Test https://doi.org/10.1507/endocrj.ej15-0695Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dae424ee0c191af5e9ac2e570560b568 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13484540 09188959 |
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