The human GINS complex binds to and specifically stimulates human DNA polymerase α‐primase
| العنوان: | The human GINS complex binds to and specifically stimulates human DNA polymerase α‐primase |
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| المؤلفون: | Mariarita De Felice, Mariarosaria De Falco, Francesca M. Pisani, Ulrich Hübscher, Mosè Rossi, Elena Ferrari |
| المساهمون: | De Falco, M, Ferrari, E, De Felice, M, Rossi, Mose', Hubscher, U, Pisani, F. M. |
| المصدر: | EMBO reports 8 (2007): 99–103. doi:10.1038/sj.embor.7400870 info:cnr-pdr/source/autori:De Falco M.; Ferrari E.; De Felice M.; Rossi M.; Hubscher U.; Pisani F.M./titolo:The human GINS complex binds and specifically stimulates human DNA polymerase alpha%2Fprimase/doi:10.1038%2Fsj.embor.7400870/rivista:EMBO reports (Print)/anno:2007/pagina_da:99/pagina_a:103/intervallo_pagine:99–103/volume:8 |
| بيانات النشر: | EMBO, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | GINS, DNA polymerase, Scientific Report, DNA replication, Biochemistry, genome dynamics, law.invention, chemistry.chemical_compound, ATP Binding Cassette Transporter, Subfamily B, Member 3, law, Escherichia coli, Genetics, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 2, Protein Precursors, Molecular Biology, Polymerase, DNA primase, biology, DNA, Surface Plasmon Resonance, DNA Polymerase I, Molecular biology, Peptide Fragments, Recombinant Proteins, Cell biology, Protein Subunits, chemistry, Recombinant DNA, biology.protein, ATP-Binding Cassette Transporters, Primase, DNA polymerase I |
| الوصف: | The eukaryotic GINS complex has an essential role in the initiation and elongation phases of genome duplication. It is composed of four paralogous subunits--Sld5, Psf1, Psf2 and Psf3--which are ubiquitous and evolutionarily conserved in eukaryotic organisms. Here, we report the biochemical characterization of the human GINS complex (hGINS). The four hGINS subunits were coexpressed in Escherichia coli in a highly soluble form and purified as a complex. hGINS was shown to interact directly with the heterodimeric human DNA primase, by using either surface plasmon resonance measurements or by immunoprecipitation experiments carried out with anti-hGINS antibodies. The DNA polymerase alpha-primase synthetic activity was specifically stimulated by hGINS on various primed DNA templates. The significance of these findings is discussed in view of the molecular dynamics at the human replication fork. |
| وصف الملف: | STAMPA |
| تدمد: | 1469-3178 1469-221X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac25169569b5d43b4bc7ff3fe2db76cfTest https://doi.org/10.1038/sj.embor.7400870Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac25169569b5d43b4bc7ff3fe2db76cf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 1469221X |
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