Extracellular vesicles deliver Mycobacterium RNA to promote host immunity and bacterial killing
| العنوان: | Extracellular vesicles deliver Mycobacterium |
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| المؤلفون: | Yong Cheng, Jeffery S. Schorey |
| المصدر: | EMBO reports. 20 |
| بيانات النشر: | EMBO, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, biology, Chemistry, RNA, Type I interferon production, biology.organism_classification, Biochemistry, Microbiology, Mycobacterium tuberculosis, 03 medical and health sciences, 0302 clinical medicine, TANK-binding kinase 1, In vivo, Genetics, IRF3, Molecular Biology, 030217 neurology & neurosurgery, 030304 developmental biology, Mycobacterium, Phagosome |
| الوصف: | Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage-derived EVs through an M.tb SecA2-dependent pathway and that EVs released from M.tb-infected macrophages stimulate a host RIG-I/MAVS/TBK1/IRF3 RNA sensing pathway, leading to type I interferon production in recipient cells. These EVs also promote, in a RIG-I/MAVS-dependent manner, the maturation of M.tb-containing phagosomes through a noncanonical LC3 pathway, leading to increased bacterial killing. Moreover, treatment of M.tb-infected macrophages or mice with a combination of moxifloxacin and EVs, isolated from M.tb-infected macrophages, significantly lowered bacterial burden relative to either treatment alone. We hypothesize that EVs, which are preferentially removed by macrophages in vivo, can be combined with effective antibiotics as a novel approach to treat drug-resistant TB. |
| تدمد: | 1469-3178 1469-221X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::0ac2fa400b536d10d486ac8882fd2ee6Test https://doi.org/10.15252/embr.201846613Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........0ac2fa400b536d10d486ac8882fd2ee6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 1469221X |
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