Radial somatic F-actin organization affects growth cone dynamics during early neuronal development
| العنوان: | Radial somatic F-actin organization affects growth cone dynamics during early neuronal development |
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| المؤلفون: | Robin Scharrenberg, Michael R. Kreutz, Carlos G. Dotti, Dennis Eggert, Oliver Kobler, Durga Praveen Meka, Froylan Calderon de Anda, Sabine Windhorst, Birgit Schwanke, Sergei Klykov, Melanie Richter, Marina Mikhaylova, Theresa König, Irina Schaefer, Bing Zhao |
| المصدر: | EMBO Reports EMBO reports, 20(12):e47743 |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Somatic cell, Neurogenesis, Cell, Growth Cones, Cell Cycle Proteins, macromolecular substances, PCM‐1, microtubules, actin, PCM-1, neuronal development, centrosome, Biology, Biochemistry, Hippocampus, Microtubules, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Microtubule, Genetics, medicine, Animals, Growth cone, Molecular Biology, Actin, Cells, Cultured, 030304 developmental biology, Centrosome, 0303 health sciences, Gene knockdown, Microtubule organizing center, Articles, Actins, Cell biology, Rats, Mice, Inbred C57BL, medicine.anatomical_structure, Cell Adhesion, Polarity & Cytoskeleton, 030217 neurology & neurosurgery, Neuroscience |
| الوصف: | The centrosome is thought to be the major neuronal microtubule‐organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin‐organizing center, raising the possibility that neuronal development may, in addition, require a centrosome‐based actin radial organization. Here, we report, using super‐resolution microscopy and live‐cell imaging of cultured rodent neurons, F‐actin organization around the centrosome with dynamic F‐actin aster‐like structures with F‐actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F‐actin stability reveal a robust flux of somatic F‐actin toward the cell periphery. Finally, we show that somatic F‐actin intermingles with centrosomal PCM‐1 (pericentriolar material 1 protein) satellites. Knockdown of PCM‐1 and disruption of centrosomal activity not only affect F‐actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F‐actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development. During early neuronal development, centrosomes regulate F‐actin organization, thereby ensuring proper growth cone dynamics and neurite length. |
| وصف الملف: | application/pdf; application/zip |
| تدمد: | 1469-3178 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0673636db508f2d86b55ba67a614b31cTest https://pubmed.ncbi.nlm.nih.gov/31650708Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0673636db508f2d86b55ba67a614b31c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 |
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