دورية أكاديمية
S-Nitrosylation of PINK1 Attenuates PINK1/Parkin-Dependent Mitophagy in hiPSC-Based Parkinson’s Disease Models
| العنوان: | S-Nitrosylation of PINK1 Attenuates PINK1/Parkin-Dependent Mitophagy in hiPSC-Based Parkinson’s Disease Models |
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| المؤلفون: | Oh, Chang-Ki, Sultan, Abdullah, Platzer, Joseph, Dolatabadi, Nima, Soldner, Frank, McClatchy, Daniel B., Diedrich, Jolene K., Ambasudhan, Rajesh, Nakamura, Tomohiro, Jaenisch, Rudolf, Lipton, Stuart A., Yates, John R., III |
| المساهمون: | Massachusetts Institute of Technology. Department of Biology, Jaenisch, Rudolf |
| المصدر: | Elsevier |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | DSpace@MIT (Massachusetts Institute of Technology) |
| الوصف: | Mutations in PARK6 (PINK1) and PARK2 (Parkin) are linked to rare familial cases of Parkinson's disease (PD). Mutations in these genes result in pathological dysregulation of mitophagy, contributing to neurodegeneration. Here, we report that environmental factors causing a specific posttranslational modification on PINK1 can mimic these genetic mutations. We describe a molecular mechanism for impairment of mitophagy via formation of S-nitrosylated PINK1 (SNO-PINK1). Mitochondrial insults simulating age- or environmental-related stress lead to increased SNO-PINK1, inhibiting its kinase activity. SNO-PINK1 decreases Parkin translocation to mitochondrial membranes, disrupting mitophagy in cell lines and human-iPSC-derived neurons. We find levels of SNO-PINK1 in brains of α-synuclein transgenic PD mice similar to those in cell-based models, indicating the pathophysiological relevance of our findings. Importantly, SNO-PINK1-mediated deficits in mitophagy contribute to neuronal cell death. These results reveal a direct molecular link between nitrosative stress, SNO-PINK1 formation, and mitophagic dysfunction that contributes to the pathogenesis of PD. Nitrosative stress and mitochondrial dysfunction represent key pathological events in Parkinson's disease. Oh et al. identify a molecular link between these events in which increased nitric oxide (NO)-related species S-nitrosylate a critical thiol group in PINK1, thus compromising its ability to eliminate damaged mitochondria via mitophagy. ; National Institutes of Health (U.S.) (Grant R01 NS086890) ; National Institutes of Health (U.S.) (Grant P01 ES016738) ; National Institutes of Health (U.S.) (Grant DP1 DA041722) ; National Institutes of Health (U.S.) (Grant RF1 AG057409) ; National Institutes of Health (U.S.) (Grant R01 AG056259) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 2211-1247 |
| العلاقة: | http://dx.doi.org/10.1016/J.CELREP.2017.10.068Test; Cell Reports; http://hdl.handle.net/1721.1/117502Test; Oh, Chang-Ki et al. “S-Nitrosylation of PINK1 Attenuates PINK1/Parkin-Dependent Mitophagy in hiPSC-Based Parkinson’s Disease Models.” Cell Reports 21, 8 (November 2017): 2171–2182 © 2017 The Authors |
| الإتاحة: | https://doi.org/10.1016/J.CELREP.2017.10.068Test http://hdl.handle.net/1721.1/117502Test |
| حقوق: | Creative Commons Attribution-NonCommercial-NoDerivs License ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.3A982F41 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22111247 |
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