دورية أكاديمية
Physiological TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case
| العنوان: | Physiological TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case |
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| المؤلفون: | Belville, Corinne, Ponelle-Chachuat, Flora, Rouzaire, Marion, Gross, Christelle, Pereira, Bruno, Gallot, Denis, Sapin, Vincent, Blanchon, Loïc |
| المساهمون: | Auvergne Rhone-Alpes Region Jeune Chercheur Framework |
| المصدر: | eLife ; volume 11 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2022 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | The integrity of human fetal membranes is crucial for harmonious fetal development throughout pregnancy. Their premature rupture is often the consequence of a physiological phenomenon that has been exacerbated. Beyond all the implied biological processes, inflammation is of primary importance and is qualified as ‘sterile’ at the end of pregnancy. In this study, complementary methylomic and transcriptomic strategies on amnion and choriodecidua explants obtained from the altered (cervix zone) and intact fetal membranes at term and before labour were used. By cross-analysing genome-wide studies strengthened by in vitro experiments, we deciphered how the expression of toll-like receptor 4 (TLR4), an actor in pathological fetal membrane rupture, is controlled. Indeed, it is differentially regulated in the altered zone and between both layers by a dual mechanism: (1) the methylation of TLR4 and miRNA promoters and (2) targeting by miRNA (let-7a-2 and miR-125b-1) acting on the 3’-UTR of TLR4. Consequently, this study demonstrates that fine regulation of TLR4 is required for sterile inflammation establishment at the end of pregnancy and that it may be dysregulated in the pathological premature rupture of membranes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.71521 |
| الإتاحة: | https://doi.org/10.7554/elife.71521Test https://cdn.elifesciences.org/articles/71521/elife-71521-v1.pdfTest https://cdn.elifesciences.org/articles/71521/elife-71521-v1.xmlTest https://elifesciences.org/articles/71521Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.89C82224 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.71521 |
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