دورية أكاديمية
Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses
| العنوان: | Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses |
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| المؤلفون: | Catsburg, Lisa AE, Westra, Manon, van Schaik, Annemarie ML, MacGillavry, Harold D |
| المساهمون: | Nederlandse Organisatie voor Wetenschappelijk Onderzoek |
| المصدر: | eLife ; volume 11 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2022 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | At postsynaptic sites of neurons, a prominent clathrin-coated structure, the endocytic zone (EZ), controls the trafficking of glutamate receptors and is essential for synaptic plasticity. Despite its importance, little is known about how this clathrin structure is organized to mediate endocytosis. We used live-cell and super-resolution microscopy to reveal the dynamic organization of this poorly understood clathrin structure in rat hippocampal neurons. We found that a subset of endocytic proteins only transiently appeared at postsynaptic sites. In contrast, other proteins were persistently enriched and partitioned at the edge of the EZ. We found that uncoupling the EZ from the synapse led to the loss of most of these components, while disrupting interactions with the actin cytoskeleton or membrane did not alter EZ positioning. Finally, we found that plasticity-inducing stimuli promoted the reorganization of the EZ. We conclude that the EZ is a stable, highly organized molecular platform where components are differentially recruited and positioned to orchestrate the endocytosis of synaptic receptors. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.74387 |
| الإتاحة: | https://doi.org/10.7554/elife.74387Test https://cdn.elifesciences.org/articles/74387/elife-74387-v2.pdfTest https://cdn.elifesciences.org/articles/74387/elife-74387-v2.xmlTest https://elifesciences.org/articles/74387Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.A917E31B |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.74387 |
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