دورية أكاديمية
Disintegration promotes protospacer integration by the Cas1-Cas2 complex
| العنوان: | Disintegration promotes protospacer integration by the Cas1-Cas2 complex |
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| المؤلفون: | Ma, Chien-Hui, Javanmardi, Kamyab, Finkelstein, Ilya J, Jayaram, Makkuni |
| المساهمون: | National Institute of General Medical Sciences, National Science Foundation, Welch Foundation |
| المصدر: | eLife ; volume 10 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2021 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | ‘Disintegration’—the reversal of transposon DNA integration at a target site—is regarded as an abortive off-pathway reaction. Here, we challenge this view with a biochemical investigation of the mechanism of protospacer insertion, which is mechanistically analogous to DNA transposition, by the Streptococcus pyogenes Cas1-Cas2 complex. In supercoiled target sites, the predominant outcome is the disintegration of one-ended insertions that fail to complete the second integration event. In linear target sites, one-ended insertions far outnumber complete protospacer insertions. The second insertion event is most often accompanied by the disintegration of the first, mediated either by the 3′-hydroxyl exposed during integration or by water. One-ended integration intermediates may mature into complete spacer insertions via DNA repair pathways that are also involved in transposon mobility. We propose that disintegration-promoted integration is functionally important in the adaptive phase of CRISPR-mediated bacterial immunity, and perhaps in other analogous transposition reactions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.65763 |
| الإتاحة: | https://doi.org/10.7554/elife.65763Test https://cdn.elifesciences.org/articles/65763/elife-65763-v1.pdfTest https://cdn.elifesciences.org/articles/65763/elife-65763-v1.xmlTest https://elifesciences.org/articles/65763Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.B22CEC85 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.65763 |
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