دورية أكاديمية
Inflammasome activation leads to cDC1-independent cross-priming of CD8 T cells by epithelial cell-derived antigen
| العنوان: | Inflammasome activation leads to cDC1-independent cross-priming of CD8 T cells by epithelial cell-derived antigen |
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| المؤلفون: | Deets, Katherine A, Nichols Doyle, Randilea, Rauch, Isabella, Vance, Russell E |
| المساهمون: | National Institutes of Health, Howard Hughes Medical Institute |
| المصدر: | eLife ; volume 10 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2021 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | The innate immune system detects pathogens and initiates adaptive immune responses. Inflammasomes are central components of the innate immune system, but whether inflammasomes provide sufficient signals to activate adaptive immunity is unclear. In intestinal epithelial cells (IECs), inflammasomes activate a lytic form of cell death called pyroptosis, leading to epithelial cell expulsion and the release of cytokines. Here, we employed a genetic system to show that simultaneous antigen expression and inflammasome activation specifically in IECs is sufficient to activate CD8 + T cells. By genetic elimination of direct T cell priming by IECs, we found that IEC-derived antigens were cross-presented to CD8 + T cells. However, cross-presentation of IEC-derived antigen to CD8 + T cells only partially depended on IEC pyroptosis. In the absence of inflammasome activation, cross-priming of CD8 + T cells required Batf3 + dendritic cells (conventional type one dendritic cells [cDC1]), whereas cross-priming in the presence of inflammasome activation required a Zbtb46 + but Batf3 -independent cDC population. These data suggest the existence of parallel inflammasome-dependent and inflammasome-independent pathways for cross-presentation of IEC-derived antigens. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.72082 |
| الإتاحة: | https://doi.org/10.7554/elife.72082Test https://cdn.elifesciences.org/articles/72082/elife-72082-v2.pdfTest https://cdn.elifesciences.org/articles/72082/elife-72082-v2.xmlTest https://elifesciences.org/articles/72082Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.FE50F611 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.72082 |
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