دورية أكاديمية
Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit
| العنوان: | Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit |
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| المؤلفون: | Ching-Ju Tsai, Jacopo Marino, Ricardo Adaixo, Filip Pamula, Jonas Muehle, Shoji Maeda, Tilman Flock, Nicholas MI Taylor, Inayatulla Mohammed, Hugues Matile, Roger JP Dawson, Xavier Deupi, Henning Stahlberg, Gebhard Schertler |
| المصدر: | eLife, Vol 8 (2019) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | G protein-coupled receptors, G proteins, cryo-EM, Gβ subunit, cellular signaling, Bos taurus, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | One of the largest membrane protein families in eukaryotes are G protein-coupled receptors (GPCRs). GPCRs modulate cell physiology by activating diverse intracellular transducers, prominently heterotrimeric G proteins. The recent surge in structural data has expanded our understanding of GPCR-mediated signal transduction. However, many aspects, including the existence of transient interactions, remain elusive. We present the cryo-EM structure of the light-sensitive GPCR rhodopsin in complex with heterotrimeric Gi. Our density map reveals the receptor C-terminal tail bound to the Gβ subunit of the G protein, providing a structural foundation for the role of the C-terminal tail in GPCR signaling, and of Gβ as scaffold for recruiting Gα subunits and G protein-receptor kinases. By comparing available complexes, we found a small set of common anchoring points that are G protein-subtype specific. Taken together, our structure and analysis provide new structural basis for the molecular events of the GPCR signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/46041Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.46041 |
| الوصول الحر: | https://doaj.org/article/e771e8ad582e45b493b49c84fa5cd9a3Test |
| رقم الانضمام: | edsdoj.771e8ad582e45b493b49c84fa5cd9a3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.46041 |